Probe:	m-eya4
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Present		7a
TS15: otocyst	Present		7a
TS15: trunk	Present	Regionally restricted	7a	Expression is in two stripes flanking the dorsal midline above the developing forelimb bud.
TS15: eye	Absent		7a

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Present		7b
TS15: otocyst	Present		7b
TS15: trunk	Present		7b	Expression is in two stripes flanking the dorsal midline above the developing forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		7c
TS17: forelimb bud	Present		7c
TS17: hindlimb bud	Present		7c
TS17: latero-nasal process	Present		7c
TS17: trunk somite	Present		7c
TS17: eye	Absent		7c

Structure	Level	Pattern	Image	Note
TS19: outer ear	Present	Regionally restricted	7d	Expression is in the future external auditory meatus.
TS19: head mesenchyme	Present	Regionally restricted	7d	Expression is in the craniofacial mesenchyme above the nose and between the eyes.
TS19: forelimb bud	Present		7d
TS19: hindlimb bud	Present		7d
TS19: trunk	Strong	Regionally restricted	7d	Expression is in the domain that links the dermomyotome with the limb.
TS19: trunk dermomyotome	Strong		7d

Structure	Level	Pattern	Image	Note
TS19: trunk dermomyotome	Strong		7e
TS19: forelimb bud	Present	Regionally restricted	7e	Expression is in streams of cells that are moving from the dermomyotome into the forelimb.
TS19: trunk	Present	Regionally restricted	7e	Expression is in streams of cells that are moving from the dermomyotome into the forelimb.
TS19: dorsal root ganglion	Absent		7e
TS19: neural tube	Absent		7e

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	7f	Expression is in the craniofacial mesenchyme overlying the olfactory epithelium.
TS19: eye	Absent		7f
TS19: lens vesicle	Absent		7f

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present	Regionally restricted	7g	Expression is around but not within the pre-cartilage condensations of the digits.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present	Regionally restricted	7h	Expression is around but not within the pre-cartilage condensations of the digits. It appears to be within the region of the developing flexor tendons.