Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80111
Yamashita T, et al., Early developmental expression of the gene encoding glucosylceramide synthase, the enzyme controlling the first committed step of glycosphingolipid synthesis. Biochim Biophys Acta. 2002 Dec 19;1573(3):236-40
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3723611
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Gene symbol:
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Ugcg
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Gene name:
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UDP-glucose ceramide glucosyltransferase
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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fixative solution was 2%
formaldehyde, 0.2% glutaraldehyde in PBS
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Results
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Specimen
3A:
embryonic day 1.0; Ugcgtm3Rlp/Ugcg +
(more  )
Note: 2 cell embryo.
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ugcgtm3Rlp/Ugcg+
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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2 cell embryo.
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Specimen
3B:
embryonic day 1.5; Ugcgtm3Rlp/Ugcg +
(more )
Note: 4 cell embryo.
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ugcgtm3Rlp/Ugcg+
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Age:
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embryonic day 1.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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4 cell embryo.
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Specimen
3C:
embryonic day 2.0; Ugcgtm3Rlp/Ugcg +
(more )
Note: 8 cell embryo.
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ugcgtm3Rlp/Ugcg+
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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8 cell embryo.
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Specimen
3D:
embryonic day 2.5; Ugcgtm3Rlp/Ugcg +
(more )
Note: Morula.
Specimen
3D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ugcgtm3Rlp/Ugcg+
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Morula.
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Specimen
3E:
embryonic day 3.5; Ugcgtm3Rlp/Ugcg +
(more )
Note: Blastocyst.
Specimen
3E:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ugcgtm3Rlp/Ugcg+
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Blastocyst.
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Specimen
3F:
embryonic day 7.5; Ugcgtm3Rlp/Ugcg +
(more )
Specimen
3F:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ugcgtm3Rlp/Ugcg+
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
Not shown:
postnatal; Ugcgtm3Rlp/Ugcg +
(more )
Note: Unfertilized oocytes.
Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ugcgtm3Rlp/Ugcg+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Unfertilized oocytes.
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Analysis Tools