Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73506
Lowe LA, et al., Genetic dissection of nodal function in patterning the mouse embryo. Development. 2001 May;128(10):1831-43
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3720878
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Embryos were fixed in 0.2% glutaraldehyde, 5mM EDTA, 2mM MgCl2.
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Results
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Specimen
1D:
embryonic day 8.5; Nodaltm1Mku/Nodal +
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
2A:
embryonic day 7.5; Nodaltm1Mku/Nodal +
(more )
Specimen
2A:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
2B:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
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2B
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Expression did not extend as far distally as in the wild type embryo.
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Specimen
2C:
embryonic day 7.5; Nodaltm1Mku/Nodal +
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: mesendoderm |
Present |
Regionally restricted |
2C
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Expression was detected in mesendodermal cells of the node.
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| TS11: node |
Present |
Regionally restricted |
2C
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Expression was detected in mesendodermal cells of the node.
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Specimen
2D:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
2D
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Expression was detected halfway down the posterior side.
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Specimen
2E:
embryonic day 7.5; Nodaltm1Mku/Nodal +
(more )
Note: Embedded in methacrylate.
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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Specimen
2F:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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Specimen
2G:
embryonic day 7.5; Nodaltm1Mku/Nodal +
(more )
Note: Embedded in methacrylate.
Specimen
2G:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: mesendoderm |
Present |
Regionally restricted |
2G
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Expression was detected in mesendoderm cells of the node.
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| TS11: node |
Present |
Regionally restricted |
2G
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Expression was detected in mesendoderm cells of the node.
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Specimen
2H:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: mesendoderm |
Present |
Regionally restricted |
2H
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Expression was detected in mesendoderm cells.
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Specimen
2I:
embryonic day 7.5; Nodaltm1Mku/Nodal +
(more )
Note: Embedded in methacrylate.
Specimen
2I:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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Specimen
2J:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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Specimen
6A, 6B:
embryonic day 8.5; Nodaltm1Mku/Nodal +
(more )
Note: Fig. 6A is a dorsal view, 6B is a view from the left side.
Specimen
6A, 6B:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Fig. 6A is a dorsal view, 6B is a view from the left side.
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Specimen
6C, 6D:
embryonic day 8.5; Nodaltm1Mku/ Nodaltm1.1Mku
(more )
Note: Type I embryo. Fig. 6C is a dorsal view, 6D is a lateral view.
Specimen
6C, 6D:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Type I embryo. Fig. 6C is a dorsal view, 6D is a lateral view.
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Specimen
6E:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Type II embryo.
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Specimen
6F:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Type III embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Present |
Regionally restricted |
6F
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Expression was detected in the presumed posterior.
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Specimen
6G:
embryonic day 8.5; Nodaltm1Mku/Nodal +
(more )
Note: Embedded in methacrylate.
Specimen
6G:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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Specimen
6H:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Type I embryo. Embedded in methacrylate.
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Specimen
6I:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Type II embryo. Embedded in methacrylate.
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Specimen
6J:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Type III embryo. Embedded in methacrylate.
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Specimen
6K:
embryonic day 8.5; Nodaltm1Mku/Nodal +
(more )
Note: Embedded in methacrylate.
Specimen
6K:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nodaltm1Mku/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
Regionally restricted |
6K
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Expression was detected around the node.
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Specimen
6L:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Type I embryo. Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
Regionally restricted |
6L
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Expression was detected around the node as in wild type.
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Specimen
6M:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Type II embryo. Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Weak |
Regionally restricted |
6M
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Staining around the node was reduced.
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Specimen
6N:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
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Note:
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Type III embryo. Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo mesenchyme |
Present |
Regionally restricted |
6N
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Expression was detected in mesodermal or mesendodermal cells in the posterior region.
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