Sox9 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122361 Svingen T, et al., Sex-specific expression of a novel gene Tmem184a during mouse testis differentiation. Reproduction. 2007 May;133(5):983-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:3714346
Gene symbol:	Sox9
Gene name:	SRY (sex determining region Y)-box 9

Probe:	Sox9-pG, Sox9-pH
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR was used to obtain these results. The graph represents three independent experiments, each performed in triplicate.

Results

Image: 4A Sox9

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
wt	E13.5	TS21: testis	Present	1 µg; total RNA	involves: CD-1		Male	3-4 wild type and heterozygous littermates were pooled for this sample.
we	E13.5	TS21: testis	Present (a)	1 µg; total RNA	involves: CD-1	Kit^W-e/Kit^W-e	Male	3-4 mutant littermates were pooled for this sample.

Notes:
(a) Expression in the mutant appears to be higher than in wild type. This is likely to be due to the increased overall proprotion of somatic cells in the absence of germ cells in the mutant gonads.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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