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Gene Expression Data
Assay Details
Assay
Reference: J:120282 de Jong H, et al., Ontogeny of mRNA expression and activity of long-chain acyl-CoA synthetase (ACSL) isoforms in Mus musculus heart. Biochim Biophys Acta. 2007 Jan;1771(1):75-82
Assay type: RT-PCR
MGI Accession ID: MGI:3712876
Gene symbol: Acsl3
Gene name: acyl-CoA synthetase long-chain family member 3
Probe: Acsl3-pA, Acsl3-pB
Probe preparation: labelled with other - see notes
Visualized with: Fluorescence
Assay notes: Quantitative real-time-PCR. Probes were dual labeled with FAM (6-carboxyfluorescein) as reporter and TAMRA (6-carboxytetramethylrhodamine) as quencher. The expression of each isoform is presented in a graph as as a percentage of the total acsl transcripts.
Results Image: 3_acsl3
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E16 E16.0 (a) TS24: heart Present (b) 0.02 µg; total RNA C57BL/6J Not Specified
P1 P1 TS27: heart Present (b) 0.02 µg; total RNA C57BL/6J Not Specified
P7 P7 TS28: heart Weak (c) 0.02 µg; total RNA C57BL/6J Not Specified
P18 P16 TS28: heart Weak 0.02 µg; total RNA C57BL/6J Not Specified
P25 P25 TS28: heart Weak 0.02 µg; total RNA C57BL/6J Not Specified
2 mo P m 2 TS28: heart Weak (d) 0.02 µg; total RNA C57BL/6J Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Acsl3 message comprised 40% of total Acsl transcripts on E16 and P1.
(c) 12%
(d) 3.5%
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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