Gene Expression Data
Assay Details
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Assay
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Reference:
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J:120282
de Jong H, et al., Ontogeny of mRNA expression and activity of long-chain acyl-CoA synthetase (ACSL) isoforms in Mus musculus heart. Biochim Biophys Acta. 2007 Jan;1771(1):75-82
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:3712876
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Gene symbol:
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Acsl3
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Gene name:
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acyl-CoA synthetase long-chain family member 3
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Probe:
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Acsl3-pA, Acsl3-pB
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Probe preparation:
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labelled with other - see notes
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Visualized with:
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Fluorescence
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Assay notes:
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Quantitative real-time-PCR. Probes were dual labeled with FAM (6-carboxyfluorescein) as reporter and
TAMRA (6-carboxytetramethylrhodamine) as quencher. The expression of each isoform is presented in a graph as as a percentage of the total acsl transcripts.
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Results
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Image: 3_acsl3
| E16.0
(a)
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TS24: heart
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Present
(b)
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0.02 µg; total RNA |
C57BL/6J |
| Not Specified |
| P1
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TS27: heart
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Present
(b)
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0.02 µg; total RNA |
C57BL/6J |
| Not Specified |
| P7
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TS28: heart
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Weak
(c)
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0.02 µg; total RNA |
C57BL/6J |
| Not Specified |
| P16
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TS28: heart
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Weak
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0.02 µg; total RNA |
C57BL/6J |
| Not Specified |
| P25
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TS28: heart
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Weak
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0.02 µg; total RNA |
C57BL/6J |
| Not Specified |
| P m 2
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TS28: heart
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Weak
(d)
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0.02 µg; total RNA |
C57BL/6J |
| Not Specified |
Notes:
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Analysis Tools
