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Gene Expression Data
Assay Details
Assay
Reference: J:119964 Acevedo N, et al., Glycogen synthase kinase-3 regulation of chromatin segregation and cytokinesis in mouse preimplantation embryos. Mol Reprod Dev. 2007 Feb;74(2):178-88
Assay type: Western blot
MGI Accession ID: MGI:3710311
Gene symbol: Gsk3a
Gene name: glycogen synthase kinase 3 alpha
Antibody: anti-phospho-GSK3 (Tyr279/216)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody used in this assay recognizes both isoforms--the phosphorylated tyrosine 279 of the alpha and the phorphorylated tyrosine 216 of the beta. The alpha isoform is 51 kDa, the beta 46-47 kDa. 80-200 oocytes and embryos were loaded per lane (and 1 ug of brain protein).
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 51 kDa (a) Genetic BackgroundMutant Allele(s)Sex
Brain P TS28: brain Present CF-1 Not Specified
Lane 2 Lane not used in this assay
MII Oocyte P (b) TS28: oocyte Present CF-1 Female
1-cell E0.5 (b) TS1: 1-cell stage conceptus Present CF-1 Not Specified
2-cell E1.0 (b) TS2: 2-cell stage conceptus Present CF-1 Not Specified
4-cell E2.0 (b) TS3: 4-cell stage conceptus Present CF-1 Not Specified
Lane 7 Lane not used in this assay
Blastocyst E3.5 (b) TS4: blastocyst-stage conceptus Present CF-1 Not Specified
Notes:
(a) This residue appears to be constitutively phorphorylated.
(b) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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