Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:59927
Guerriero A, et al., PU.1 is required for myeloid-derived but not lymphoid-derived dendritic cells. Blood. 2000 Feb 1;95(3):879-85
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3710310
|
|
Gene symbol:
|
Ly75
|
|
Gene name:
|
lymphocyte antigen 75
|
|
Antibody:
|
Anti-DEC-205
|
|
Detection system:
|
Secondary antibody coupled to alkaline phosphatase
|
|
Assay notes:
|
Wild type embryos could have been either PU.1+/+ or PU.1+/-.
|
|
|
Results
|
Specimen
3 E14.5 WT:
embryonic day 14.5
(more  )
Specimen
3 E14.5 WT:
(close  )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thymus primordium |
Present |
Regionally restricted |
3_E14.5 WT
|
Expression was mainly restricted to the subcapsular cortex of the thymus.
|
Specimen
3 E14.5 PU.1-/-:
(close )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Mutant Allele(s):
|
Spi1tm1Sing/Spi1tm1Sing
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thymus primordium |
Weak |
|
3_E14.5 PU.1-/-
|
Expression pattern in the mutant was less organized than the wild type.
|
Specimen
3 E16.5 WT:
embryonic day 16.5
(more )
Specimen
3 E16.5 WT:
(close )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thymus |
Present |
Regionally restricted |
3_E16.5 WT
|
Expression was mainly restricted to the subcapsular cortex of the thymus.
|
Specimen
3 E16.5 PU.1-/-:
(close )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Mutant Allele(s):
|
Spi1tm1Sing/Spi1tm1Sing
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thymus |
Present |
|
3_E16.5 PU.1-/-
|
Expression levels were near wild type. Expression pattern in the mutant was less organized than the wild type.
|
Specimen
4 E14.5 WT:
embryonic day 14.5
(more )
Note: The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
Specimen
4 E14.5 WT:
(close )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
|
Specimen
4 E14.5 PU.1-/-:
embryonic day 14.5; Spi1tm1Sing/ Spi1tm1Sing
(more )
Note: The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
Specimen
4 E14.5 PU.1-/-:
(close )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Mutant Allele(s):
|
Spi1tm1Sing/Spi1tm1Sing
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thymus primordium |
Weak |
|
4_DEC-205 PU.1-/- E14.5
|
Expression level in the mutant was less than 10% of wild type.
|
Specimen
4 E16.5 WT:
embryonic day 16.5
(more )
Note: The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
Specimen
4 E16.5 WT:
(close )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thymus |
Present |
|
4_DEC-205 WT E16.5
|
|
Specimen
4 E16.5 PU.1-/-:
embryonic day 16.5; Spi1tm1Sing/ Spi1tm1Sing
(more )
Note: The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
Specimen
4 E16.5 PU.1-/-:
(close )
|
Genetic Background:
|
involves: 129S/SvEv
|
|
Mutant Allele(s):
|
Spi1tm1Sing/Spi1tm1Sing
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thymus |
Present |
|
4_DEC-205 PU.1-/- E16.5
|
Expression level in the mutant was approximately 70% of wild type.
|
|
Analysis Tools