Gsk3a Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:119964 Acevedo N, et al., Glycogen synthase kinase-3 regulation of chromatin segregation and cytokinesis in mouse preimplantation embryos. Mol Reprod Dev. 2007 Feb;74(2):178-88
Assay type:	Western blot
MGI Accession ID:	MGI:3710262
Gene symbol:	Gsk3a
Gene name:	glycogen synthase kinase 3 alpha

Antibody:	anti-GSK3
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The antibody used in this assay recognizes both isoforms. The alpha isoform is 51 kDa, the beta 46-47 kDa. 80-200 oocytes and embryos were loaded per lane (and 1 ug of brain protein).

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	51 kDa	Genetic Background	Mutant Allele(s)	Sex
Brain	P	TS28: brain	Present (a)	CF-1		Not Specified
Lane 2	Lane not used in this assay
MII Oocyte	P (b)	TS28: oocyte	Present (c)	CF-1		Female
1-cell	E0.5 (b)	TS1: 1-cell stage conceptus	Present (c)	CF-1		Not Specified
2-cell	E1.0 (b)	TS2: 2-cell stage conceptus	Present (c)	CF-1		Not Specified
4-cell	E2.0 (b)	TS3: 4-cell stage conceptus	Present (c)	CF-1		Not Specified
Lane 7	Lane not used in this assay
Blastocyst	E3.5 (b)	TS4: blastocyst-stage conceptus	Present (a)	CF-1		Not Specified

Notes:
(a) The expression levels of the two isoforms are equivalent in this sample.
(b) Age assigned by curator based on morphological criteria supplied by authors.
(c) The alpha isoform is expressed at twice the level of the beta isoform.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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