Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(Pomc1-hrGFP)1Lowl
Name:	transgene insertion 1, Bradford B Lowell
MGI ID:	MGI:3709191
Synonyms:	POMC-GFP, POMC-hrGFP
Transgene:	Tg(Pomc1-hrGFP)1Lowl  Location: unknown
Alliance:	Tg(Pomc1-hrGFP)1Lowl page

Strain of Origin:

FVB

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence in-frame with the ATG transcriptional start site of the mouse pro-opiomelanocortin-alpha (Pomc1) gene (this also deleted the first 30 nucleotides of Pomc1). Correctly transformed and targeted bacteria were used to isolate the 150 kb BAC, which was then injected into the pronucleus of fertilized one-cell stage FVB embryos. Transgenic line 1 was subsequently established. When exposed to UV light, brain tissue shows GFP fluorescence patterns consistent with endogenous Pomc1 expression. (J:120992, J:125165)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:120992 Lowell BB, Generation of humanized Renilla GFP transgenic reporter mice. MGI Direct Data Submission. 2007;
All:	27 reference(s)