Probe:	AC8 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Absent			There is no expression in the retinal ganglion cells.
TS23: cortical plate	Weak	Regionally restricted		There is weak expression in cortical layer V. There is no expression in layers IV and VI.
TS23: telencephalon	Moderate	Non-Uniform		There is moderate expression in the medial septum. There is weak expression in the subiculum and globus pallidus. There is no expression in hippocampal areas CA1, CA2, and CA3, the anterior amygdala, or the central medial and basomedial nuclei.
TS23: olfactory lobe	Weak
TS23: corpus striatum	Absent
TS23: thalamus	Weak	Non-Uniform		There is weak expression in the dorsal and ventral lateral geniculate nucleus and reticularus. There is none in the subthalamus and the ventrobasal, ventrolateral, posterior, and anteroventral nuclei.
TS23: epithalamus	Strong	Regionally restricted		There is strong expression in the habenula.
TS23: hypothalamus	Strong	Non-Uniform		There is strong expression in the supraoptic region. There is moderate expression in the hypothalamus and preoptic region. There is no expression in the acurate nucleus.
TS23: midbrain	Strong	Regionally restricted		There is strong expression in the superior colliculus. There is no expression in the inferior colliculus and substantia nigra.
TS23: pons	Strong	Non-Uniform		There is strong expression in the pontine nucleus. There is weak expression in the locus coeruleus and raphe nucleus. There is no expression in the trigeminal and cochlear nuclei.
TS23: medulla oblongata	Strong	Regionally restricted		There is strong expression in the inferior olive.
TS23: cerebellum	Moderate			There is moderate expression in the Purkinje cells and the deep cerebellar nucleus.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Weak
TS28: cortical layer II	Weak
TS28: retina ganglion cell layer	Weak
TS28: cortical layer III	Moderate
TS28: cortical layer IV	Strong
TS28: cortical layer V	Weak
TS28: cortical layer VI	Weak
TS28: dentate gyrus	Absent
TS28: hippocampus CA2	Absent
TS28: hippocampus CA3	Absent
TS28: hippocampus CA1	Weak
TS28: hippocampus	Present	Regionally restricted		Although there is expression elsewhere in the hippocampus, there is no expression in the hippocampal interneurons.
TS28: subiculum	Weak
TS28: main olfactory bulb	Moderate
TS28: amygdala	Moderate	Non-Uniform		There is moderate expression in the basomedial nuclei and weak expression in the anterior amygdala.
TS28: central medial thalamic nucleus	Moderate
TS28: corpus striatum	Absent
TS28: medial septal complex	Moderate
TS28: globus pallidus	Moderate
TS28: posterior thalamic nucleus	Strong			There is strong expression in the posterior nucleus.
TS28: anterior ventral thalamic nucleus	Weak			There is weak expression in the ventrobasal, ventrolateral, and anteroventral nuclei.
TS28: basal ventral medial thalamic nucleus	Weak			There is weak expression in the ventrobasal, ventrolateral, and anteroventral nuclei.
TS28: ventral posterior lateral thalamic nucleus	Weak			There is weak expression in the ventrobasal, ventrolateral, and anteroventral nuclei.
TS28: lateral geniculate nucleus	Moderate	Non-Uniform		There is moderate expression in the ventral lateral geniculate and weak expression in the dorsal lateral geniculate.
TS28: reticular thalamic nucleus	Weak
TS28: subthalamic nucleus	Absent
TS28: habenula	Strong
TS28: hypothalamus	Strong	Regionally restricted		There is strong expression in the supraoptic region, moderate expression in the preoptic region, and weak expression in the hypothalamus.
TS28: arcuate nucleus	Absent
TS28: superior colliculus	Strong
TS28: inferior colliculus	Absent
TS28: substantia nigra	Absent
TS28: locus coeruleus	Weak
TS28: raphe nucleus	Moderate
TS28: pontine nucleus	Weak
TS28: inferior olivary nucleus	Moderate
TS28: trigeminal nucleus	Absent
TS28: cochlear nucleus	Weak
TS28: cerebellum Purkinje cell layer	Moderate
TS28: deep cerebellar nucleus	Moderate
TS28: cerebellum granule cell layer	Absent

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Moderate
TS28: cortical layer I	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Strong
TS28: cortical layer V	Moderate
TS28: cortical layer VI	Moderate
TS28: dentate gyrus	Absent
TS28: hippocampus CA1	Moderate
TS28: hippocampus CA2	Absent
TS28: hippocampus CA3	Absent
TS28: hippocampus	Present	Regionally restricted		Although there is expression elsewhere in the hippocampus, there is no expression in the hippocampal interneurons.
TS28: subiculum	Weak
TS28: main olfactory bulb	Strong
TS28: amygdala	Moderate	Non-Uniform		There is moderate expression in the basomedial nucleus, and no expression in the anterior amygdala.
TS28: central medial thalamic nucleus	Weak
TS28: corpus striatum	Absent
TS28: medial septal complex	Moderate
TS28: globus pallidus	Moderate
TS28: basal ventral medial thalamic nucleus	Moderate			There is moderate expression in the ventrobasal nucleus.
TS28: anterior ventral thalamic nucleus	Weak			There is weak expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: posterior thalamic nucleus	Weak			There is weak expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: ventral posterior lateral thalamic nucleus	Weak			There is weak expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: lateral geniculate nucleus	Moderate			There is moderate expression in both the dorsal and ventral lateral geniculate nucleus.
TS28: habenula	Moderate
TS28: arcuate nucleus	Absent
TS28: superior colliculus	Strong
TS28: inferior colliculus	Absent
TS28: substantia nigra	Absent
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum granule cell layer	Absent
TS28: deep cerebellar nucleus	Moderate

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Moderate
TS28: cortical layer I	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Weak
TS28: cortical layer V	Strong
TS28: cortical layer VI	Moderate
TS28: dentate gyrus	Absent			In the table the annotation for this structure is ambiguous, but in the legend for Fig. 6 the authors discuss the lack of expression in the dentate gyrus.
TS28: hippocampus CA1	Moderate
TS28: hippocampus CA2	Absent
TS28: hippocampus CA3	Absent
TS28: hippocampus	Present	Regionally restricted		Although there is expression elsewhere in the hippocampus, there is no expression in the hippocampal interneurons.
TS28: subiculum	Absent
TS28: amygdala	Moderate	Non-Uniform		There is moderate expression in the basomedial nuclei, and weak expression in the anterior amygdala.
TS28: central medial thalamic nucleus	Moderate
TS28: corpus striatum	Absent
TS28: globus pallidus	Moderate
TS28: basal ventral medial thalamic nucleus	Strong			There is strong expression in the ventrobasal nucleus.
TS28: anterior ventral thalamic nucleus	Moderate			There is moderate expression in the posterior and anteroventral nuclei.
TS28: posterior thalamic nucleus	Moderate			There is moderate expression in the posterior and anteroventral nuclei.
TS28: ventral posterior lateral thalamic nucleus	Weak			There is weak expression in the ventrolateral nucleus.
TS28: lateral geniculate nucleus	Strong	Non-Uniform		There is strong expression in the dorsal lateral geniculate and moderate expression in the ventral lateral geniculate.
TS28: reticular thalamic nucleus	Weak
TS28: subthalamic nucleus	Absent
TS28: habenula	Strong
TS28: hypothalamus	Strong	Non-Uniform		There is strong expression in the supraoptic area. There is moderate expression in the hypothalamus and preoptic area.
TS28: superior colliculus	Strong
TS28: inferior colliculus	Absent
TS28: substantia nigra	Absent
TS28: locus coeruleus	Moderate
TS28: raphe nucleus	Moderate
TS28: pontine nucleus	Weak
TS28: inferior olivary nucleus	Strong
TS28: cerebellum Purkinje cell layer	Moderate
TS28: deep cerebellar nucleus	Moderate
TS28: cerebellum granule cell layer	Absent

Structure	Level	Pattern	Image	Note
TS23: olfactory lobe	Present		3G

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present	Regionally restricted	3H	Expression is present in postmigratory neurons in the lateralmost portions of the plate.
TS23: cerebral cortex subventricular zone	Absent		3H
TS23: cerebral cortex ventricular layer	Absent		3H
TS23: telencephalon	Present	Regionally restricted	3H	Expression is present in the nucleus basalis of Meynert and the corticobasal nuclei of the amygdala.

Structure	Level	Pattern	Image	Note
TS23: hypothalamus	Present	Regionally restricted	3I	Expression in present in the magnocellular hypothalamic cell groups, in the supraoptic nuclei, and the paraventricular hypothalamic nuclei, as well as the preoptic area.
TS23: telencephalon	Present	Regionally restricted	3I	Expression is present in the pallidum.
TS23: corpus striatum	Absent		3I

Structure	Level	Pattern	Image	Note
TS23: epithalamus	Present		4C	Expression is present in the epithalamus, including the habenula and the anlage of the pineal gland.

Structure	Level	Pattern	Image	Note
TS23: epithalamus	Present		4F	Expression is present in the epithalamus, including the habenula and the anlage of the pineal gland.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present		4I	Expression is present in the deep cerebellar nuclei and Purkinje cell primordia.
TS23: midbrain	Strong	Regionally restricted	4I	There is strong expression in the tectum and in the superficial layers of the superior colliculus.
TS23: hindbrain	Strong	Regionally restricted	4I	There is strong expression in the dorsal rhombic lip.

Structure	Level	Pattern	Image	Note
TS27: lateral geniculate nucleus	Present		6A	Expression is present in the dorsal lateral geniculate.
TS27: basal ventral medial thalamic nucleus	Present		6A	Expression is present in the ventrobasal nucleus.
TS27: neocortex layer	Present	Regionally restricted	6A	Expression is limited to the subplate and to the deep cortical layers.

Structure	Level	Pattern	Image	Note
TS28: lateral geniculate nucleus	Present		6B	Expression is present in the dorsal lateral geniculate.
TS28: basal ventral medial thalamic nucleus	Present		6B	Expression is present in the ventrobasal nucleus.
TS28: neocortex layer	Present		6B	Expression is present in the upper cortical layers.
TS28: hippocampus CA1	Present		6B
TS28: dentate gyrus	Absent		6B
TS28: hippocampus CA2	Absent		6B
TS28: hippocampus CA3	Absent		6B

Structure	Level	Pattern	Image	Note
TS28: lateral geniculate nucleus	Strong		6C	There is strong expression in the dorsal lateral geniculate.
TS28: basal ventral medial thalamic nucleus	Strong	Regionally restricted	6C	There is strong expression in the ventrobasal nucleus.
TS28: neocortex layer	Present		6C	Expression is present in the upper cortical layers. It is strongest in layer V, moderate to low in II, II, IV, and VIb, and very low in VIa.
TS28: cingulate cortex	Strong		6C	There is strong expression in the anterior and posterior cingulate gyrus.
TS28: hippocampus CA1	Present		6C
TS28: piriform cortex	Present		6C
TS28: dentate gyrus	Absent		6C
TS28: hippocampus CA2	Absent		6C
TS28: hippocampus CA3	Absent		6C

Structure	Level	Pattern	Image	Note
TS27: cortical layer IV	Strong	Regionally restricted	7A , 7D , 7C	There is dense expression in the primary somatosensory cortex.
TS27: neocortex region	Strong	Regionally restricted	7A , 7D , 7C	There is dense expression in the primary somatosensory cortex.
TS27: medial septal complex	Strong	Regionally restricted	7A	There is dense expression in the medial septal nuclei.
TS27: amygdala	Present	Regionally restricted	7A	There is expression in the corticobasal nuclei and in the nucleus basalis of Meynert.
TS27: forebrain	Present	Regionally restricted	7A	There is expression in the basal forebrain.

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Strong	Regionally restricted	7B	There is dense expression in the primary somatosensory cortex.
TS28: neocortex region	Strong	Regionally restricted	7B	There is dense expression in the primary somatosensory cortex.
TS28: forebrain	Present	Regionally restricted	7B	There is expression in the basal forebrain.
TS28: medial septal complex	Strong	Regionally restricted	7B	There is dense expression in the medial septal nuclei.
TS28: cingulate cortex	Strong		7B

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		8A
TS28: deep cerebellar nucleus	Present		8A
TS28: superior colliculus	Strong	Regionally restricted	8A	There is strong expression in the superficial layers.
TS28: tectum	Strong		8A

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Present		8B' , 8B
TS27: main olfactory bulb mitral cell layer	Present		8B' , 8B
TS27: retina ganglion cell layer	Absent		8B , 8B'

Structure	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus	Present		8C
TS28: cerebellum Purkinje cell layer	Weak		8C
TS28: dorsal tegmental nucleus	Strong		8C
TS28: cochlear nucleus	Strong		8C	There is strong expression in the anteroventral cochlear nucleus.
TS28: cerebellum granule cell layer	Absent		8C

Structure	Level	Pattern	Image	Note
TS28: locus coeruleus	Present		8D
TS28: raphe nucleus	Present		8D

Structure	Level	Pattern	Image	Note
TS21: epithalamus	Present			Expression is present in the epithalamus, including the habenula and the anlage of the pineal gland.
TS21: hindbrain	Strong	Regionally restricted		Expression is present in the dorsal rhombic lip.