Probe:	AC1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	At all times expression appears to be restricted to postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Present	Non-Uniform	5A	Tangential sections of the area reveal that there is intense neuropil staining in the center of the barrels.
TS28: corpus striatum	Present		5A
TS28: cortical layer V	Present		5A
TS28: lateral septal complex	Present	Regionally restricted	5A	Expression is present in the dorsolateral septal nuclei.
TS28: inferior colliculus	Strong		5A

Structure	Level	Pattern	Image	Note
TS28: reticular thalamic nucleus	Present		5B	This is the area rostral thalamus with the strongest staining.
TS28: anterior thalamic group	Present		5B	Expression is present in the anterodorsal thalamic groups.
TS28: habenula	Absent		5B
TS28: inferior colliculus	Strong		5B
TS28: pineal gland	Absent		5B

Structure	Level	Pattern	Image	Note
TS28: basal ventral medial thalamic nucleus	Strong		5C	There is strong staining in the nuclei derived from the dorsal thalamus--the dorsal lateral geniculate nucleus, the ventrobasal nucleus, and the posterior nucleus.
TS28: lateral geniculate nucleus	Strong		5C	There is strong staining in the nuclei derived from the dorsal thalamus--the dorsal lateral geniculate nucleus, the ventrobasal nucleus, and the posterior nucleus.
TS28: posterior thalamic nucleus	Strong		5C	There is strong staining in the nuclei derived from the dorsal thalamus--the dorsal lateral geniculate nucleus, the ventrobasal nucleus, and the posterior nucleus.
TS28: habenula	Absent		5C
TS28: pineal gland	Absent		5C

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Regionally restricted	5D	There is intense expression in the hippocampal interneurons.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Regionally restricted	5E	There is intense expression in the hippocampal interneurons.

Structure	Level	Pattern	Image	Note
TS28: inferior olivary nucleus	Strong		5F
TS28: trigeminal nucleus	Strong	Regionally restricted	5F
TS28: spinal cord dorsal horn	Strong		5F	Expression is present in the spinal subnuclei oralis, interpolaris, and caudalis.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		5G	Expression is present in the neurons of the preplate, in the marginal zone of the cortical mantle, extending throughout the telencephalon.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		5H	Expression is present in the neurons of the preplate, in the marginal zone of the cortical mantle, extending throughout the telencephalon.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong	Regionally restricted	5I	There is strong expression in the neurons.
TS21: spinal cord	Strong	Regionally restricted	5I	There is strong expression in the neurons.
TS21: thalamus	Present		5I	Expression is present in all dorsal and ventral thalamic derivatives.
TS21: hypothalamus	Absent		5I	There is no expression in hypothalamic derivatives.
TS21: epithalamus	Absent		5I	There is no expression in epithalmic derivatives.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Strong