Probe:	PQBP-1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS18: embryo	Weak		1_E11

Structure	Level	Pattern	Image	Note
TS21: embryo	Weak		1_E13

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present		1_E15

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present		1_E18

Structure	Level	Pattern	Image	Note
TS23: brain	Present		2A	Expression is distributed throughout the central nervous system.
TS23: cerebral cortex	Strong		2A	The cerebral cortex and hippocampus show higher signals than other regions.
TS23: telencephalon	Strong	Regionally restricted	2A	The cerebral cortex and hippocampus show higher signals than other regions.
TS23: brainstem	Present		2A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Strong		2B
TS23: cortical plate	Strong		2B

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	2C	There is no expression in the peripheral white matter, suggesting neurons have a higher expression level than glia.

Structure	Level	Pattern	Image	Note
TS23: corpus striatum	Present	Regionally restricted	2D	There is staining in the striatum, but there are distinctive streaks that do not stain.

Structure	Level	Pattern	Image	Note
TS23: eye	Weak		2E
TS23: eyelid	Weak	Regionally restricted	2E	Expression is present in connective tissues around the palpebral cleft.

Structure	Level	Pattern	Image	Note
TS23: heart	Absent		3A

Structure	Level	Pattern	Image	Note
TS23: lung	Absent		3B

Structure	Level	Pattern	Image	Note
TS23: liver	Absent		3C

Structure	Level	Pattern	Image	Note
TS23: intestine	Absent		3D

Structure	Level	Pattern	Image	Note
TS23: foot skin	Present		3E
TS23: foot mesenchyme	Absent		3E	The only appreciable expression in the foot is in the skin.
TS23: hindlimb digit	Absent		3E	The only appreciable expression in the foot is in the skin.
TS23: hindlimb interdigital region	Absent		3E	The only appreciable expression in the foot is in the skin.

Structure	Level	Pattern	Image	Note
TS23: adrenal cortex	Present	Regionally restricted	3F	Expression is present in the peripheral region.
TS23: metanephros	Absent		3F

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong		4_P1
TS27: brain	Present		4_P1

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong		4_P5
TS28: brain	Present		4_P5

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		4_P10
TS28: hippocampus	Present		4_P10
TS28: cerebral cortex	Trace		4_P10

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		4_Adult
TS28: hippocampus	Present		4_Adult
TS28: cerebral cortex	Absent		4_Adult

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		5A
TS27: hippocampus	Present		5A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak		5B

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Trace		5C
TS28: hippocampus	Present		5C

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Strong		5D
TS28: cerebral cortex	Present		5D

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Weak		5E

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong		6A
TS27: cerebellum internal granule cell layer	Strong		6A

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong		6B
TS28: cerebellum internal granule cell layer	Strong		6B

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		6C

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Weak		6D
TS28: cerebellum Purkinje cell layer	Absent		6D
TS28: cerebellum molecular layer	Absent		6D

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted		Expression is present in the cerebral cortex and the hippocampus. It is not present in the striatum.
TS18: cerebellum primordium	Present	Regionally restricted		Expression is present in the Purkinje cells. It is not present in the external granular layer.
TS18: brain	Present	Regionally restricted		Although expression is present elsewhere in the brain, it is not present in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present
TS21: corpus striatum	Present
TS21: telencephalon	Present	Regionally restricted		Expression is present in the hippocampus.
TS21: cerebellum	Present	Regionally restricted		Expression is present in the Purkinje cell layer. It is not present in the external granular layer.
TS21: cerebral cortex subventricular zone	Absent

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present
TS23: cerebral cortex subventricular zone	Present
TS23: corpus striatum	Present
TS23: telencephalon	Present	Regionally restricted		Expression is present in the hippocampus.
TS23: cerebellum	Present	Regionally restricted		Expression is present in the Purkinje cell layer and the granular cell layer. It is not present in the molecular layer nor the external granule cell layer.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present
TS26: cerebral cortex subventricular zone	Present
TS26: corpus striatum	Present
TS26: hippocampus	Present
TS26: cerebellum	Present	Regionally restricted		Expression is present in the Purkinje cell layer and the granule cell layer. It is not present it in the molecular layer. It may or may not be present in the external granule cell layer.

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Present
TS27: cerebellum external granule cell layer	Present
TS27: cerebellum granule cell layer	Present
TS27: cerebral cortex	Present
TS27: corpus striatum	Present
TS27: hippocampus	Present
TS27: cerebellum Purkinje cell layer	Absent
TS27: cerebellum molecular layer	Absent

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present
TS28: cerebellum external granule cell layer	Present
TS28: cerebellum granule cell layer	Present
TS28: cerebral cortex	Present
TS28: corpus striatum	Present
TS28: hippocampus	Present
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum molecular layer	Absent

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present
TS28: cerebellum external granule cell layer	Present
TS28: cerebellum granule cell layer	Present
TS28: cerebral cortex	Present
TS28: corpus striatum	Present
TS28: hippocampus	Present
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum molecular layer	Absent

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum external granule cell layer	Absent
TS28: cerebral cortex	Absent
TS28: corpus striatum	Absent
TS28: brain subventricular zone	Present
TS28: cerebellum granule cell layer	Present
TS28: hippocampus	Present