Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Tg(KRT14-RAF1/ESR1)1Pkha
Name:	transgene insertion 1, Paul A Khavari
MGI ID:	MGI:3700057
Synonyms:	K14-Raf:ER
Transgene:	Tg(KRT14-RAF1/ESR1)1Pkha  Location: unknown
Alliance:	Tg(KRT14-RAF1/ESR1)1Pkha page

Strain of Origin:

C57BL/6

Transgene Type:    Transgenic (Constitutively active, Humanized sequence, Inducible, Inserted expressed sequence) Inducer:    tamoxifen Mutation:    Insertion   Tg(KRT14-RAF1/ESR1)1Pkha expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human RAF1 (5894) Raf1  (MGI:97847)   Mutation details: The transgene contains a 2075-bp human keratin 14 promoter construct driving expression of a cDNA that encodes a fusion protein comprising a constitutively active mutant form of the catalytic domain of human RAF1, in which tyrosine is replaced by aspartic acid at amino acid positions 340 and 341 (Y340D/Y341D), joined at its carboxyl-terminal to the ligand-binding domain of human estrogen receptor 1. The fusion protein can be activated in the epidermis by topical application of 4-hydroxytamoxifen. (J:81660, J:118584, J:118585)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:81660 Tarutani M, et al., Inducible activation of ras and raf in adult epidermis. Cancer Res. 2003 Jan 15;63(2):319-23
All:	5 reference(s)