Bcat1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:117809 Castellano S, et al., Expression of cytosolic branched chain aminotransferase (BCATc) mRNA in the developing mouse brain. Gene Expr Patterns. 2007 Feb;7(4):485-90
Assay type:	RNA in situ
MGI Accession ID:	MGI:3699345
Gene symbol:	Bcat1
Gene name:	branched chain aminotransferase 1, cytosolic

Probe:	BCATc cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1A ©

Specimen 1A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Diffuse	1A

1B ©

Specimen 1B: embryonic day 12.5 (more

)

Structure	Level	Image
TS20: hindbrain	Strong	1B
TS20: midbrain	Strong	1B
TS20: telencephalon	Strong	1B

1D ©

Specimen 1D: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong		1D
TS20: cerebral cortex marginal layer	Absent		1D

1E ©

Specimen 1E: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	1E	Expression is in the ventral mesencephalon.

1F ©

Specimen 1F: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Strong	Regionally restricted	1F	Expression is in the ventricular zone.

1G ©

Specimen 1G: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	1G	Expression is in the medial longitudinal fasciculus.

1H ©

Specimen 1H: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: brain	Present		1H

1I ©

Specimen 1I: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Diffuse	1I

1J ©

Specimen 1J: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		1J
TS22: cortical plate	Present		1J

1K ©

Specimen 1K: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Very strong	Regionally restricted	1K	Expression is at the level of the external germinal layer.

1L ©

Specimen 1L: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	1L	Expression is in the pontine nuclei.

1M ©

Specimen 1M: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata lateral wall	Present	Regionally restricted	1M	Expression is in the raphe nuclei.

1N ©

Specimen 1N: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	1N	Expression is in the inferior olive.

Specimen Table 1 E12.5: embryonic day 12.5 (more

)

Structure	Level	Pattern	Note
TS20: midbrain	Strong	Regionally restricted	Expression is strong in the ventral mesencephalon, weak in the dorsal part.
TS20: cerebral cortex	Moderate
TS20: forebrain	Moderate	Regionally restricted	Expression is moderate in the basal forebrain and is weak in the hippocampus.
TS20: cerebellum primordium	Weak
TS20: epithalamus	Weak
TS20: hypothalamus	Weak
TS20: thalamus	Weak
TS20: medulla oblongata	Weak	Regionally restricted	Expression is weak in the locus coeruleus and medial longitudinal fasciculus, absent in raphe nuclei and the inferior olive.
TS20: pons	Absent		Expression is absent in the pontine nuclei.

Specimen Table 1 E14.5: embryonic day 14.5 (more

)

Structure	Level	Pattern	Note
TS22: medulla oblongata	Strong	Regionally restricted	Expression is in the raphe nuclei and the inferior olive and is weak in the locus coeruleus and medial longitudinal fasciculus.
TS22: pons	Strong	Regionally restricted	Expression is in the pontine nuclei.
TS22: cerebellum	Moderate
TS22: cerebral cortex	Moderate
TS22: forebrain	Moderate	Regionally restricted	Expression is moderate in the basal forebrain and is weak in the hippocampus.
TS22: epithalamus	Weak
TS22: hypothalamus	Weak
TS22: midbrain	Weak
TS22: thalamus	Weak

Specimen Table 1 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: medulla oblongata	Strong	Regionally restricted	Expression is in the raphe nuclei and the inferior olive and is weak in the locus coeruleus and medial longitudinal fasciculus.
TS24: pons	Strong	Regionally restricted	Expression is in the pontine nuclei.
TS24: cerebellum	Moderate
TS24: cerebral cortex	Moderate
TS24: forebrain	Moderate	Regionally restricted	Expression is moderate in the basal forebrain and is weak in the hippocampus.
TS24: epithalamus	Weak
TS24: hypothalamus	Weak
TS24: midbrain	Weak
TS24: thalamus	Weak

2A ©

Specimen 2A: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: brain	Present		2A

2C ©

Specimen 2C: postnatal day 0 (more

)

Structure	Level	Image
TS27: cortical layer II	Strong	2C
TS27: cortical layer III	Strong	2C
TS27: cortical layer IV	Strong	2C
TS27: cortical layer V	Strong	2C
TS27: cortical layer VI	Strong	2C

2D ©

Specimen 2D: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Strong		2D
TS27: hippocampus CA3	Strong		2D

2E ©

Specimen 2E: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: substantia nigra pars compacta	Strong		2E

2F ©

Specimen 2F: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong		2F

2G ©

Specimen 2G: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: raphe nucleus	Strong		2G

2H ©

Specimen 2H: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2H

2J ©

Specimen 2J: postnatal day 7 (more

)

Structure	Level	Image
TS28: cortical layer II	Present	2J
TS28: cortical layer III	Present	2J
TS28: cortical layer V	Present	2J
TS28: cortical layer VI	Present	2J
TS28: cortical layer IV	Weak	2J

2K ©

Specimen 2K: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		2K

2L ©

Specimen 2L: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Present		2L
TS28: substantia nigra pars reticulata	Present		2L

2M ©

Specimen 2M: postnatal day 7 (more

)

Structure	Level	Image
TS28: cerebellum Purkinje cell layer	Very strong	2M
TS28: cerebellum external granule cell layer	Very strong	2M
TS28: cerebellum internal granule cell layer	Present	2M
TS28: cerebellum molecular layer	Absent	2M

2N ©

Specimen 2N: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: raphe nucleus	Strong		2N

3A ©

Specimen 3A: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: brain	Present		3A

3C ©

Specimen 3C: postnatal day 21 (more

)

Structure	Level	Image
TS28: cortical layer II	Present	3C
TS28: cortical layer III	Present	3C
TS28: cortical layer V	Present	3C
TS28: cortical layer VI	Present	3C
TS28: cortical layer IV	Weak	3C

3D ©

Specimen 3D: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		3D

3E ©

Specimen 3E: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: reticular thalamic nucleus	Strong		3E

3F ©

Specimen 3F: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Strong		3F

3G ©

Specimen 3G: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		3G

3H ©

Specimen 3H: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Present		3H

3I ©

Specimen 3I: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: raphe nucleus	Strong		3I

3L ©

Specimen 3L: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: inferior olivary nucleus	Strong		3L

Specimen Table 2 P0: postnatal day 0 (more

)

Structure	Level	Pattern	Note
TS27: hippocampus CA3	Strong
TS27: inferior olivary nucleus	Strong
TS27: pontine nucleus	Strong
TS27: raphe nucleus	Strong
TS27: cerebellum external granule cell layer	Moderate
TS27: cortical layer II	Moderate
TS27: cortical layer III	Moderate
TS27: cortical layer IV	Moderate
TS27: cortical layer V	Moderate
TS27: cortical layer VI	Moderate
TS27: hippocampus CA1	Moderate
TS27: amygdala	Weak
TS27: anterior thalamic group	Weak
TS27: cerebellum Purkinje cell layer	Weak
TS27: cerebellum internal granule cell layer	Weak
TS27: cerebellum molecular layer	Weak
TS27: corpus striatum	Weak
TS27: dentate gyrus	Weak
TS27: habenula	Weak
TS27: hypothalamus	Weak
TS27: lateral geniculate nucleus	Weak
TS27: locus coeruleus	Weak
TS27: reticular thalamic nucleus	Weak
TS27: substantia nigra pars compacta	Weak
TS27: substantia nigra pars reticulata	Weak
TS27: superior colliculus	Weak
TS27: telencephalon	Weak	Regionally restricted	Expression is in the septum.
TS27: lateral thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS27: medial thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS27: midbrain tegmentum	Weak	Regionally restricted	Expression is ventral.
TS27: cortical layer I	Absent

Specimen Table 2 P7: postnatal day 7 (more

)

Structure	Level	Pattern	Note
TS28: anterior thalamic group	Strong
TS28: habenula	Strong
TS28: hippocampus CA3	Strong
TS28: inferior olivary nucleus	Strong
TS28: pontine nucleus	Strong
TS28: raphe nucleus	Strong
TS28: reticular thalamic nucleus	Strong
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum external granule cell layer	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cortical layer V	Moderate
TS28: cortical layer VI	Moderate
TS28: dentate gyrus	Moderate
TS28: hippocampus CA1	Moderate
TS28: substantia nigra pars compacta	Moderate
TS28: amygdala	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: corpus striatum	Weak
TS28: cortical layer IV	Weak
TS28: hypothalamus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: locus coeruleus	Weak
TS28: substantia nigra pars reticulata	Weak
TS28: superior colliculus	Weak
TS28: lateral thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS28: medial thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS28: midbrain tegmentum	Weak	Regionally restricted	Expression is ventral.
TS28: telencephalon	Weak		Expression is in the septum.
TS28: cerebellum molecular layer	Absent
TS28: cortical layer I	Absent

Specimen Table 2 P21: postnatal day 21 (more

)

Structure	Level	Pattern	Note
TS28: anterior thalamic group	Strong
TS28: cerebellum Purkinje cell layer	Strong
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong
TS28: habenula	Strong
TS28: hippocampus CA3	Strong
TS28: inferior olivary nucleus	Strong
TS28: pontine nucleus	Strong
TS28: raphe nucleus	Strong
TS28: reticular thalamic nucleus	Strong
TS28: cerebellum internal granule cell layer	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: dentate gyrus	Moderate
TS28: hippocampus CA1	Moderate
TS28: substantia nigra pars compacta	Moderate
TS28: telencephalon	Moderate	Regionally restricted	Expression is in the septum.
TS28: amygdala	Weak
TS28: corpus striatum	Weak
TS28: cortical layer IV	Weak
TS28: hypothalamus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: locus coeruleus	Weak
TS28: substantia nigra pars reticulata	Weak
TS28: superior colliculus	Weak
TS28: lateral thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS28: medial thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS28: midbrain tegmentum	Weak	Regionally restricted	Expression is ventral.
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum molecular layer	Absent
TS28: cortical layer I	Absent

Specimen Table 2 Adult: postnatal adult (more

)

Structure	Level	Pattern	Note
TS28: anterior thalamic group	Strong
TS28: cerebellum Purkinje cell layer	Strong
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong
TS28: habenula	Strong
TS28: hippocampus CA3	Strong
TS28: inferior olivary nucleus	Strong
TS28: pontine nucleus	Strong
TS28: raphe nucleus	Strong
TS28: reticular thalamic nucleus	Strong
TS28: substantia nigra pars compacta	Strong
TS28: cerebellum internal granule cell layer	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: dentate gyrus	Moderate
TS28: hippocampus CA1	Moderate
TS28: substantia nigra pars reticulata	Moderate
TS28: telencephalon	Moderate	Regionally restricted	Expression is in the septum.
TS28: midbrain tegmentum	Moderate	Regionally restricted	Expression is ventral.
TS28: amygdala	Weak
TS28: corpus striatum	Weak
TS28: cortical layer IV	Weak
TS28: hypothalamus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: locus coeruleus	Weak
TS28: superior colliculus	Weak
TS28: lateral thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS28: medial thalamic group	Weak	Regionally restricted	Expression is in the ventrolateral/ventromedial thalamic nucleus.
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum molecular layer	Absent
TS28: cortical layer I	Absent

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