Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67360
Caruana G, et al., Craniofacial dysmorphogenesis including cleft palate in mice with an insertional mutation in the discs large gene. Mol Cell Biol. 2001 Mar;21(5):1475-83
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3698945
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Gene symbol:
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Dlg1
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Gene name:
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discs large MAGUK scaffold protein 1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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The authors state that identical results were obtained with homozygous mutant embryos (-/-).
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Results
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Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Regionally restricted |
2B
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Expression is in vasculature.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Regionally restricted |
2C
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Expression is in blood cells, at regions of cell contact in endothelial cells, and at the basal and lateral membranes of the epithelial cells of the endoderm.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2D':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2D'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
2I
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Expression is in the epithelial dermomyotome component at the face adjacent to the scleratome.
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
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2J
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Expression is at the apical membrane of epithelial cells.
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Specimen
2K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: metanephros |
Present |
Regionally restricted |
2K
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Expression is at all membranes of epithelial cells.
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Specimen
2L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: gut |
Present |
Regionally restricted |
2L
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Expression is at all membranes of epithelial cells.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5A':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: 1st branchial arch |
Present |
Regionally restricted |
5A'
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Expression is at the apical membrane of the epithelium of the first branchial arch.
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1Gt(gt1.8geo)1Ber
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1Gt(gt1.8geo)1Ber
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: palatal shelf mesenchyme |
Present |
Graded |
5F
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Prior to fusion there are low levels of expression in the tips of the shelves with higher expression adjacent to this region.
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| TS23: nasal cavity epithelium |
Strong |
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5F
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Expression is in the pseudostratified ciliated columnar epithelium.
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| TS23: oral epithelium |
Strong |
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5F
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Expression is in the stratified squamous epithelium.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: palatal shelf epithelium |
Present |
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5G
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Expression is in medial-edge epithelial cells at the time of palatal-shelf fusion.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Dlg1Gt(gt1.8geo)1Ber/Dlg1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Analysis Tools
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