Probe:	Lrrtm4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	4A	Expression is in the caudal lateral mesoderm.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	4B	Expression is in the caudal lateral mesoderm.

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	4C	Expression is in the caudal mesoderm.
TS15: limb mesenchyme	Present		4C
TS15: neural tube	Present		4C
TS15: head	Present	Regionally restricted	4C	Expression is in three discrete regions.
TS15: future midbrain	Present	Regionally restricted	4C	Expression is in the ventral midbrain.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	4D	Expression is in the ventral midbrain and adjacent to the forebrain in the lamina terminalis.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Regionally restricted	4E	Expression is in the ventral midbrain and adjacent to the forebrain in the lamina terminalis.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	4F	Expression is in the distal limb bud.

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud mesenchyme	Present	Regionally restricted	4G	Expression is in the distal limb bud.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present		4H
TS16: limb ectoderm	Absent		4H

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present	Regionally restricted	4I	Caudal mesoderm expression is located ventral to the presomitic mesoderm.
TS15: tail neural tube	Present	Regionally restricted	4I	Expression is restricted to ventro-lateral cells dorsal to the floor plate.

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	4J	Expression in the rostral neural tube is restricted to ventro-lateral cells of the motor horn.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	4K	Expression is in the head mesenchyme on either side of Rathke's pouch.

Structure	Level	Pattern	Image	Note
TS18: tail sclerotome	Present	Regionally restricted	4L	Expression is in stripes in the anterior mesoderm that are localized to a subset of cells in the developing sclerotome.
TS18: trunk sclerotome	Present	Regionally restricted	4L	Expression is in stripes in the anterior mesoderm that are localized to a subset of cells in the developing sclerotome.
TS18: hindlimb bud mesenchyme	Weak	Regionally restricted	4L	Expression is in a wedge-shaped domain in the lateral mesenchyme of the hindlimb bud.
TS18: tail mesenchyme	Present	Regionally restricted	4L	Expression is in the ventral mesoderm posterior to the hind limbs.
TS18: neural tube	Present		4L
TS18: tail neural tube	Present		4L
TS18: forelimb bud mesenchyme	Absent		4L

Structure	Level	Pattern	Image	Note
TS18: neural tube	Present	Regionally restricted	4M	Expression is in the rostral neural tube in ventro-lateral cells.

Structure	Level	Pattern	Image	Note
TS18: neural tube	Present	Regionally restricted	4N	Expression is in the rostral neural tube in ventro-lateral cells.

Structure	Level	Pattern	Image	Note
TS18: trunk sclerotome	Present	Regionally restricted	4O	Expression is in stripes in the anterior mesoderm that are localized to a subset of cells in the developing sclerotome.
TS18: neural tube	Present	Regionally restricted	4O	Expression is in central lateral cells of the interlimb level neural tube.

Structure	Level	Pattern	Image	Note
TS18: tail sclerotome	Present	Regionally restricted	4P
TS18: tail mesenchyme	Present	Regionally restricted	4P	Expression is in the ventral mesoderm posterior to the hind limbs.

Structure	Level	Pattern	Image	Note
TS18: trunk somite	Present	Regionally restricted	4Q	Expression in less mature somites can be seen to extend the entire width of the somite, which then restricts to a stripe in the anterior half of the somite.
TS18: hindlimb bud mesenchyme	Weak	Regionally restricted	4Q	Expression is in a wedge-shaped domain in the lateral mesenchyme of the hindlimb bud.
TS18: forelimb bud mesenchyme	Absent		4Q

Structure	Level	Pattern	Image	Note
TS18: trunk somite	Present	Regionally restricted	4R	Expression in less mature somites can be seen to extend the entire width of the somite, which then restricts to a stripe in the anterior half of the somite.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	4S	Expression is in a wedge-shaped domain in the lateral mesenchyme of the hindlimb bud and is stronger dorsally.
TS17: tail sclerotome	Present	Regionally restricted	4S	Expression is in stripes in the anterior mesoderm that are localized to a subset of cells in the developing sclerotome.
TS17: trunk sclerotome	Present	Regionally restricted	4S	Expression is in stripes in the anterior mesoderm that are localized to a subset of cells in the developing sclerotome.
TS17: neural tube	Present	Regionally restricted	4S	Expression is in central lateral cells of the interlimb level neural tube.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	4T	Expression is in a wedge-shaped domain in the lateral mesenchyme of the hindlimb bud.
TS17: tail mesenchyme	Present	Regionally restricted	4T	Expression is in the ventral mesoderm posterior to the hind limbs.