Probe:	Dact3 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The validity of tissue expression observed for each gene was further confirmed by the observation of identical expression patterns using multiple nonoverlapping antisense probes derived from the same cDNA (corresponding to nucleotides 813-1153, 1162-1641, and 1643-1910).

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme	Present		2C
TS14: dorsal aorta	Strong	Regionally restricted	2C	Expression is in the aortic sac.
TS14: 1st arch artery	Strong		2C
TS14: 2nd arch artery	Strong		2C
TS14: branchial arch mesenchyme	Strong		2C
TS14: trunk somite	Present	Regionally restricted	2C	Expression is in the ventral domain of more mature somites, located centrally along the rostral/caudal axis.
TS14: trunk unsegmented mesenchyme	Absent		2C

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme	Strong		2F
TS17: limb mesenchyme	Strong		2F
TS17: trunk somite	Strong	Regionally restricted	2F	Expression is in maturing somites.

Structure	Level	Pattern	Image	Note
TS14: branchial arch mesenchyme	Strong		2P
TS14: head mesenchyme	Strong		2P
TS14: dorsal aorta	Strong	Regionally restricted	2P	Expression is in the aortic sac.

Structure	Level	Pattern	Image	Note
TS17: trunk somite	Strong	Regionally restricted	2Q	Expression is in the ventral domain of more mature somites, located centrally along the rostral/caudal axis.
TS17: tail unsegmented mesenchyme	Absent		2Q

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Strong		2R

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme	Strong		2S

Structure	Level	Pattern	Image	Note
TS22: brain	Present		3C
TS22: spinal cord	Present		3C

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		3F

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3Q
TS28: hippocampus	Present		3Q

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		3Q inset