Probe:	Dact2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The expression patterns was validated with a probe that corresponds to nucleotides 310-1080.

Structure	Level	Pattern	Image	Note
TS14: eye	Present	Regionally restricted	2B	Expression is in the retina.
TS14: otic placode	Present		2B
TS14: umbilical vein	Present		2B
TS14: umbilical vein extraembryonic part	Present		2B
TS14: foregut	Present	Regionally restricted	2B	Expression is in the ventral mesentery.
TS14: neural tube	Present	Regionally restricted	2B	Expression is in the dorsal neural tube.
TS14: trunk somite	Present	Graded	2B	The highest levels are dorsolaterally in the caudal (youngest) somites.
TS14: trunk unsegmented mesenchyme	Absent		2B

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Graded	2B left inset	The highest levels are dorsolaterally in the caudal (youngest) somites and becomes more rostrally restricted.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	2B right inset	Expression is in the dorsal neural tube caudally.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2E	Expression is in the caudal-most somites and in the most recently-formed somite, a weaker signal is in the next two youngest somites.
TS17: otocyst	Present		2E

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2E inset	Expression is in the caudal-most somites and in the most recently-formed somite, a weaker signal is in the next two youngest somites.

Structure	Level	Pattern	Image	Note
TS14: otic placode	Present		2M
TS14: umbilical vein	Present		2M
TS14: umbilical vein extraembryonic part	Present		2M
TS14: foregut	Present	Regionally restricted	2M	Expression is in the ventral mesentery.

Structure	Level	Pattern	Image	Note
TS14: umbilical vein	Present		2O
TS14: umbilical vein extraembryonic part	Present		2O
TS14: trunk somite	Present	Regionally restricted	2O	Rostral and dorsal expression.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Strong		3B
TS22: salivary gland	Strong		3B

Structure	Level	Pattern	Image	Note
TS22: forebrain	Weak	Regionally restricted	3E	Expression is in either the proliferative zones or postmitotic domains of the forebrain.

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Strong		3K
TS22: spinal cord	Present		3K
TS22: sympathetic ganglion	Present		3K

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Strong		3L
TS22: renal pelvis	Strong		3L

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Strong		3M

Structure	Level	Pattern	Image	Note
TS22: salivary gland	Present		3N
TS22: tooth	Weak		3N

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3P
TS28: hippocampus	Present		3P

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		3P inset