Tfap2c RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:113442 Winger Q, et al., Analysis of transcription factor AP-2 expression and function during mouse preimplantation development. Biol Reprod. 2006 Sep;75(3):324-33
Assay type:	RT-PCR
MGI Accession ID:	MGI:3689861
Gene symbol:	Tfap2c
Gene name:	transcription factor AP-2, gamma

Probe:	AP-2-gamma pG, AP-2-gamma pH
Visualized with:	Ethidium bromide
Assay notes:	Nested primers were used for this experiment. The outer set was MGI:3689827. This experiment took advantage of a polymorphism that occurs between the FVB/NJ and C57BL/6J strains to determine whether the expression observed in two-cell embryos was due to maternally inherited transcripts or due to zygotic transcription.

Results

Image: 1C left

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
M	Mol. Wt. Marker Lane
FVB-AvaII	E1.5 (a)	TS2: 2-cell stage conceptus	Present	Not Specified	FVB/NJ		Not Specified
FVB-AlwNI	E1.5 (a)	TS2: 2-cell stage conceptus	Present (b)	Not Specified	FVB/NJ		Not Specified
FVBxC57-AvaII	E1.5 (a)	TS2: 2-cell stage conceptus	Present (c)	Not Specified	(FVB/NJ x C57BL/6J)F1		Not Specified
FVBxC57-AlwNI	E1.5 (a)	TS2: 2-cell stage conceptus	Present	Not Specified	(FVB/NJ x C57BL/6J)F1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The presence of a 137 bp digested product in this lane indicates that maternally inherited RNA is still present at this age.
(c) The presence of a 92 bp digested product in this lane indicates that zygotic transcription from the paternal allele has begun.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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