Probe:	mshisa cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS8: anterior visceral endoderm	Present		2A

Structure	Level	Pattern	Image	Note
TS9: anterior visceral endoderm	Present		2B

Structure	Level	Pattern	Image	Note
TS10: anterior visceral endoderm	Present		2C

Structure	Level	Pattern	Image	Note
TS10: definitive endoderm	Present	Regionally restricted	2D	Expression is in the anterior definitive endoderm.
TS10: embryo mesoderm	Present	Regionally restricted	2D	Expression is the cranial mesoderm beneath the anterior definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2E	Expression is in the anterior definitive endoderm.
TS11: embryo mesoderm	Present	Regionally restricted	2E	Expression is the cranial mesoderm beneath the anterior definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2E'	Expression is in the anterior definitive endoderm.
TS11: embryo mesoderm	Present	Regionally restricted	2E'	Expression is the cranial mesoderm beneath the anterior definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2E''	Expression is in the anterior definitive endoderm.
TS11: embryo mesoderm	Present	Regionally restricted	2E''	Expression is the cranial mesoderm beneath the anterior definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	2E'''	Expression is in the anterior definitive endoderm.
TS11: embryo mesoderm	Present	Regionally restricted	2E'''	Expression is the cranial mesoderm beneath the anterior definitive endoderm.

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		2F
TS11: mesendoderm	Present	Regionally restricted	2F	Although it is present elsewhere, expression is absent in the midline axial mesendoderm.

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		2F'
TS11: future spinal cord neural plate	Present		2F'

Structure	Level	Pattern	Image	Note
TS12: future brain neural fold	Present		2G

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum endoderm	Present		2G'
TS12: future prosencephalon	Present		2G'
TS12: head mesenchyme	Present		2G'

Structure	Level	Pattern	Image	Note
TS12: notochordal plate	Present	Regionally restricted	2G''	Expression is at the rostral end.

Structure	Level	Pattern	Image	Note
TS13: eye	Present		2H
TS13: future prosencephalon	Present		2H
TS13: somite	Present		2H

Structure	Level	Pattern	Image	Note
TS13: eye	Present		2H'

Structure	Level	Pattern	Image	Note
TS13: optic pit	Present		2H''
TS13: surface ectoderm	Present		2H''
TS13: otic placode	Present	Regionally restricted	2H''	Expression is in the lateral region.

Structure	Level	Pattern	Image	Note
TS14: eye	Present		2I
TS14: prosencephalon	Present		2I
TS14: somite	Present		2I

Structure	Level	Pattern	Image	Note
TS14: optic eminence	Present		2I'
TS14: optic vesicle	Present		2I'
TS14: somite	Present		2I'

Structure	Level	Pattern	Image	Note
TS14: dermomyotome	Present		2I''
TS14: optic eminence	Present		2I''
TS14: optic vesicle	Present		2I''

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		2J
TS15: eye	Present		2J
TS15: future forebrain	Present		2J
TS15: trunk somite	Present		2J

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present		2J'
TS15: foregut	Present	Regionally restricted	2J'	Expression is in the ventral endoderm of the foregut.
TS15: trunk mesenchyme	Present	Regionally restricted	2J'	Expression is in the mesenchyme adjacent to the ventral endoderm of the foregut.
TS15: future forebrain	Present	Regionally restricted	2J'	Expression is in the neuroepithelium of the prosencephalic vesicles.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2K	Expression is in the dorsal telencephalon.
TS19: eye	Present		2K
TS19: tail somite	Present		2K
TS19: trunk somite	Present		2K
TS19: limb	Present	Regionally restricted	2K	Expression is in a proximal domain.

Structure	Level	Pattern	Image	Note
TS19: tail dermomyotome	Present		2K'

Structure	Level	Pattern	Image	Note
TS19: tail unsegmented mesenchyme	Absent		2K''

Structure	Level	Pattern	Image	Note
TS19: limb	Present	Regionally restricted	2L	Expression is in the distal tip.

Structure	Level	Pattern	Image	Note
TS19: limb ectoderm	Present		2L'

Structure	Level	Pattern	Image	Note
TS21: limb digit	Present	Regionally restricted	2M	Expression is at the tips.