Probe:	Grhl2 cDNA1
Probe preparation:	Antisense labelled with P33 RNA

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Strong	Uniform	1B	Expression was detected widely throughout the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Strong		1E

Structure	Level	Pattern	Image	Note
TS20: skin	Strong		1H

Structure	Level	Pattern	Image	Note
TS23: skin	Weak	Regionally restricted	1K	Expression was detected in the basal layer, stratum spinosum and stratum granulosum.

Structure	Level	Pattern	Image	Note
TS25: skin	Weak	Regionally restricted	1N	Expression was detected in the basal layer, stratum spinosum and stratum granulosum.

Structure	Level	Pattern	Image	Note
TS24: skin	Weak	Regionally restricted	1Q	Expression was detected in the basal layer, stratum spinosum and stratum granulosum.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted		Expression was detected in surface ectoderm and oesophagus but was absent from: submandibular/sublingual glands, heart, lung, kidney, olfactory epithelium, oral epithelium, stomach, midgut, hindgut and urogenital tract.
TS13: surface ectoderm	Strong
TS13: oral region	Absent			Expression was absent from oral epithelium.
TS13: genitourinary system	Absent
TS13: heart	Absent
TS13: hindgut diverticulum	Absent
TS13: midgut	Absent

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted		Expression was detected in surface ectoderm, olfactory epithelium, oral epithelium, oesophagus, midgut, hindgut, and urogenital tract/bladder but was absent from: submandibular/sublingual glands, heart, lung, kidney, and stomach.
TS17: midgut	Strong
TS17: olfactory epithelium	Strong
TS17: oral epithelium	Strong
TS17: surface ectoderm	Strong
TS17: esophagus	Present
TS17: genitourinary system	Present
TS17: hindgut	Present
TS17: heart	Absent
TS17: lung	Absent
TS17: stomach	Absent
TS17: oral region gland	Absent			Expression was absent from submandibular/sublingual glands.

Structure	Level	Pattern	Image	Note
TS20: lung	Strong
TS20: midgut	Strong
TS20: olfactory epithelium	Strong
TS20: oral epithelium	Strong
TS20: skin	Strong
TS20: urinary system	Strong			Expression was strong in the urogenital tract/bladder.
TS20: esophagus	Present
TS20: hindgut	Present
TS20: metanephros	Present
TS20: gland	Absent			Expression was absent from submandibular/sublingual glands.
TS20: heart	Absent
TS20: stomach	Absent

Structure	Level	Pattern	Image	Note
TS23: bladder	Strong			Expression was strong in the urogenital tract/bladder.
TS23: reproductive system	Strong			Expression was strong in the urogenital tract/bladder.
TS23: hindgut	Present
TS23: lung	Present
TS23: metanephros	Present
TS23: midgut	Present
TS23: olfactory epithelium	Present
TS23: oral epithelium	Present
TS23: esophagus	Weak
TS23: skin	Weak
TS23: stomach	Weak
TS23: heart	Absent
TS23: sublingual gland primordium	Absent
TS23: submandibular gland primordium	Absent

Structure	Level	Pattern	Image	Note
TS25: sublingual gland	Strong
TS25: submandibular gland	Strong
TS25: bladder	Strong			Expression was strong in the urogenital tract/bladder.
TS25: reproductive system	Strong			Expression was strong in the urogenital tract/bladder.
TS25: metanephros	Present
TS25: olfactory epithelium	Present
TS25: oral epithelium	Present
TS25: duodenum	Present			Expression was detected in midgut/small intestine.
TS25: ileum	Present			Expression was detected in midgut/small intestine.
TS25: jejunum	Present			Expression was detected in midgut/small intestine.
TS25: anal region	Present			Expression was detected in the hindgut/large intestine.
TS25: colon	Present			Expression was detected in the hindgut/large intestine.
TS25: rectum	Present			Expression was detected in the hindgut/large intestine.
TS25: esophagus	Weak
TS25: lung	Weak
TS25: skin	Weak
TS25: stomach	Weak
TS25: heart	Absent

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		2B

Structure	Level	Pattern	Image	Note
TS24: oral epithelium	Present		2E
TS24: tongue	Present	Regionally restricted	2E	Expression was detected in the lining of the tongue.

Structure	Level	Pattern	Image	Note
TS23: esophagus	Weak		2H

Structure	Level	Pattern	Image	Note
TS25: stomach	Weak		2K

Structure	Level	Pattern	Image	Note
TS24: colon	Present		2N	Expression was detected in distal hindgut.
TS24: bladder	Present	Regionally restricted	2N	Expression was detected in the epithelial lining.
TS24: ureter	Present	Regionally restricted	2N	Expression was detected in the epithelial lining.
TS24: urethra of male	Present	Regionally restricted	2N	Expression was detected in the epithelial lining.

Structure	Level	Pattern	Image	Note
TS20: lung	Present		4B

Structure	Level	Pattern	Image	Note
TS22: lung	Present		4E

Structure	Level	Pattern	Image	Note
TS23: lung	Present		4H

Structure	Level	Pattern	Image	Note
TS24: bronchiole	Strong		4K
TS24: main bronchus	Strong		4K
TS24: trachea	Strong		4K

Structure	Level	Pattern	Image	Note
TS25: lung	Weak	Regionally restricted	4N	Expression was low in the lung parenchyma.

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Present	Regionally restricted	5B	Expression was detected in the epithelial lining.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Present	Regionally restricted	5E	Expression was detected in the epithelial lining.

Structure	Level	Pattern	Image	Note
TS24: collecting duct	Present	Regionally restricted	5H	Expression was detected in the epithelial lining of the branches of the ureteric bud.

Structure	Level	Pattern	Image	Note
TS25: collecting duct	Present	Regionally restricted	5K	Expression was detected in the epithelial lining of the branches of the ureteric bud.