Probe:	Cdca4 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Present		3A
TS13: head mesenchyme	Present		3A
TS13: trunk unsegmented mesenchyme	Present		3A

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		3B
TS15: head mesenchyme	Present		3B
TS15: mesonephros	Present		3B

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted		Expression was detected in the neuroepithelium.
TS15: future hindbrain	Present	Regionally restricted		Expression was detected in the neuroepithelium.
TS15: future midbrain	Present	Regionally restricted		Expression was detected in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Strong		3C
TS17: 1st branchial arch maxillary component	Strong		3C
TS17: 2nd branchial arch	Strong		3C
TS17: fronto-nasal process	Strong		3C
TS17: future spinal cord	Present		3C
TS17: hindbrain	Present		3C
TS17: midbrain	Present		3C
TS17: trunk somite	Present		3C
TS17: dorsal root ganglion	Weak		3C
TS17: forelimb bud	Strong	Regionally restricted	3C	Expression was detected in distal mesenchyme of the fore- and hindlimb and was also detected at both the anterior and posterior edges of the proximal limb buds.
TS17: hindlimb bud	Strong	Regionally restricted	3C	Expression was detected in distal mesenchyme of the fore- and hindlimb and was also detected at both the anterior and posterior edges of the proximal limb buds.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		3D
TS19: hindbrain	Present		3D
TS19: midbrain	Present		3D
TS19: trunk somite	Present		3D
TS19: dorsal root ganglion	Weak		3D
TS19: forelimb bud	Strong	Regionally restricted	3D	Expression was detected in distal mesenchyme of the fore- and hindlimb and was also detected at both the anterior and posterior edges of the proximal limb buds.
TS19: hindlimb bud	Strong	Regionally restricted	3D	Expression was detected in distal mesenchyme of the fore- and hindlimb and was also detected at both the anterior and posterior edges of the proximal limb buds.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		3E
TS19: hindbrain	Present		3E
TS19: midbrain	Present		3E
TS19: trunk somite	Present		3E
TS19: dorsal root ganglion	Weak		3E

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region	Present		3F
TS20: vibrissa	Present		3F

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	3G	Expression was restricted dorsally.
TS19: dorsal root ganglion	Present		3G

Structure	Level	Pattern	Image	Note
TS21: limb interdigital region	Present	Regionally restricted	3H	Expression flanked the phalanges of the digits in both anterior and posterior domains and was particularly concentrated at the site of future joints.

Structure	Level	Pattern	Image	Note
TS22: integumental system	Present	Regionally restricted	3I	Expression was detected in hair follicles across the entire body of the embryo.
TS22: limb interdigital region	Present	Regionally restricted	3I	Expression flanked the phalanges of the digits in both anterior and posterior domains.