Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Casttm1Tcs
Name:	calpastatin; targeted mutation 1, Takaomi C Saido
MGI ID:	MGI:3641275
Gene:	Cast  Location: Chr13:74840487-74956929 bp, - strand  Genetic Position: Chr13, 40.52 cM, cytoband C1
Alliance:	Casttm1Tcs page

Germline Transmission:	Earliest citation of germline transmission: J:109976
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Most of exon 6 was replaced with a pgk-neo in reverse orientation. Northern and Western blot analysis confirmed recombination of the locus, as did RT-PCR. Calpastatin activity assays indicated that calpastatin activity was reduced to an undetectable level in the mutant mouse brain. (J:109976)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cast Mutation:	62 strains or lines available

Original:	J:109976 Takano J, et al., Calpain mediates excitotoxic DNA fragmentation via mitochondrial pathways in adult brains: evidence from calpastatin mutant mice. J Biol Chem. 2005 Apr 22;280(16):16175-84
All:	13 reference(s)