Gene Expression Data
Assay Details
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Assay
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Reference:
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J:107704
Xiang M, Requirement for Brn-3b in early differentiation of postmitotic retinal ganglion cell precursors. Dev Biol. 1998 May 15;197(2):155-69
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3641274
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Gene symbol:
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Pou4f3
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Gene name:
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POU domain, class 4, transcription factor 3
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Antibody:
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anti-Brn-3c
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
1I:
embryonic day 11.5
(more  )
Specimen
1I:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1J:
embryonic day 12.5
(more )
Specimen
1J:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1K:
embryonic day 13.5
(more )
Specimen
1K:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
1K Anti-Brn-3c
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Expression was detected in differentiated ganglion cells in the ganglion cell layer.
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Specimen
1L:
embryonic day 15.5
(more )
Specimen
1L:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
1L Anti-Brn-3c
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Expression was detected in differentiated ganglion cells in the ganglion cell layer.
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Specimen
1U:
embryonic day 16.5
(more )
Specimen
1U:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina |
Present |
Regionally restricted |
1U Anti-Brn-3c
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Expression was detected in differentiated ganglion cells in the ganglion cell layer.
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Specimen
1V:
embryonic day 19.5
(more )
Specimen
1V:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 19.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: retina |
Present |
Regionally restricted |
1V Anti-Brn-3c
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Expression was detected in differentiated ganglion cells in the ganglion cell layer. Severalfold more cells were labeled in the late embryonic retina than in the early postnatal retina.
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Specimen
1W:
postnatal day 0
(more )
Specimen
1W:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1X:
postnatal day 5
(more )
Specimen
1X:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina ganglion cell layer |
Present |
Regionally restricted |
1X Anti-Brn-3c
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Expression was detected in differentiated ganglion cells. Severalfold more cells were labeled in the late embryonic retina than in the early postnatal retina.
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Specimen
3K:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3L:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5I:
embryonic day 13.5; Pou4f2tm1Nat/Pou4f2 +
(more )
Specimen
5I:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
5I Anti-Brn3c
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Expression was detected in ganglion cells.
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Specimen
5M:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
5M Anti-Brn3c
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Specimen
5J:
embryonic day 15.5; Pou4f2tm1Nat/Pou4f2 +
(more )
Specimen
5J:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
5J Anti-Brn3c
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Expression was detected in ganglion cells.
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Specimen
5N:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
5N Anti-Brn3c
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Specimen
5K:
embryonic day 16.5; Pou4f2tm1Nat/Pou4f2 +
(more )
Specimen
5K:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
|
section
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|
Fixation:
|
Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina |
Present |
Regionally restricted |
5K Anti-Brn3c
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Expression was detected in ganglion cells.
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Specimen
5O:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
Paraformaldehyde
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|
Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina |
Present |
Regionally restricted |
5O Anti-Brn3c
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Specimen
5L:
embryonic day 18.5; Pou4f2tm1Nat/Pou4f2 +
(more )
Specimen
5L:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: retina |
Present |
Regionally restricted |
5L Anti-Brn3c
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Expression was detected in ganglion cells.
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Specimen
5P:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Nat/Pou4f2tm1Nat
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: retina |
Present |
Regionally restricted |
5P Anti-Brn3c
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There were 4-6 fold fewer labeled cells in the mutant retina compared to control.
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Analysis Tools