Antibody:	ALK antibody
Detection system:	Secondary antibody coupled to Alexa Fluor 555

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present
TS17: 2nd branchial arch	Present
TS17: 4th ventricle	Present
TS17: facial ganglion	Present
TS17: lens pit	Present
TS17: surface ectoderm	Present
TS17: head mesenchyme	Present	Single cells		Expression is in scattered cells in the cephalic mesenchyme.
TS17: diencephalon	Present	Regionally restricted		Expression is in a specific area and in scattered cells in neuroepithelium.
TS17: telencephalon	Present	Single cells		Expression is in scattered cells in the wall.
TS17: neural tube lateral wall	Present	Regionally restricted		Expression is in the marginal layer, mantle layer and in neural extensions.
TS17: trigeminal ganglion	Present	Regionally restricted		Expression is in the rootlets and isolated cells in the ganglion.
TS17: dorsal root ganglion	Present	Regionally restricted		Expression is in the neural extensions.
TS17: olfactory epithelium	Present	Regionally restricted		Expression is in isolated cells throughout.
TS17: heart	Present	Single cells		Expression is in scattered cells.
TS17: liver	Present	Single cells		Expression is in scattered cells.
TS17: optic cup	Present	Regionally restricted		Expression is in the neural layer and the pigment layer of the retina.
TS17: eye	Present	Regionally restricted		Expression is in the region of the optic chiasm.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present
TS19: 2nd branchial arch	Present
TS19: 4th ventricle	Present
TS19: facial ganglion	Present
TS19: lens vesicle	Present
TS19: neural retinal epithelium	Present
TS19: neural tube mantle layer	Present
TS19: neural tube marginal layer	Present
TS19: pigmented retinal epithelium	Present
TS19: surface ectoderm	Present
TS19: head mesenchyme	Present	Single cells		Expression is in scattered cells in the cephalic mesenchyme.
TS19: telencephalon	Present	Single cells		Expression is in scattered cells in the wall.
TS19: trigeminal ganglion	Present	Regionally restricted		Expression is in the rootlets and isolated cells in the ganglion.
TS19: dorsal root ganglion	Present	Regionally restricted		Expression is in the neural extensions.
TS19: olfactory epithelium	Present	Regionally restricted		Expression is in isolated cells throughout.
TS19: heart	Present	Single cells		Expression is in scattered cells.
TS19: liver	Present	Single cells		Expression is in scattered cells.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present
TS20: medulla oblongata	Present
TS20: midbrain	Present
TS20: neural retinal epithelium	Present
TS20: optic chiasma	Present
TS20: pigmented retinal epithelium	Present
TS20: pons	Present
TS20: skin	Present
TS20: spinal cord mantle layer	Present
TS20: spinal cord marginal layer	Present
TS20: tongue	Present
TS20: vagal nerve trunk	Present
TS20: brain	Present	Single cells		Expression is in scattered cells.
TS20: liver	Present	Single cells		Expression is in scattered cells.
TS20: diencephalon	Present	Regionally restricted		Expression is in part of the outer lining, in a broad band in the dorsal wall, and in scattered cells in neuroepithelium.
TS20: telencephalon	Present	Single cells		Expression is in scattered cells in the wall.
TS20: facial ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS20: trigeminal ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS20: vestibulo-cochlear ganglion complex	Present	Regionally restricted		Expression is in the rootlets.
TS20: spinal cord	Present	Regionally restricted		Expression is in the neural extensions.
TS20: utricle	Present	Regionally restricted		Expression is in the semicircular canal.
TS20: nasal cavity epithelium	Present	Regionally restricted		Expression is in isolated cells in the most caudal part.
TS20: heart	Present	Single cells		Expression is in scattered cells in the complete heart.
TS20: intestine	Present	Regionally restricted		Expression is in cells surrounding the edges.
TS20: skeletal muscle	Present	Regionally restricted		Expression is in an area of lung muscle in the neck.

Structure	Level	Pattern	Image	Note
TS21: cartilage	Present
TS21: cerebral cortex	Present
TS21: dorsal root ganglion	Present
TS21: esophagus	Present
TS21: lateral ventricle choroid plexus	Present
TS21: lens	Present
TS21: medulla oblongata	Present
TS21: metencephalon part of 4th ventricle choroid plexus	Present
TS21: midbrain	Present
TS21: neural retinal epithelium	Present
TS21: olfactory lobe	Present
TS21: optic chiasma	Present
TS21: optic nerve	Present
TS21: pigmented retinal epithelium	Present
TS21: pons	Present
TS21: skin	Present
TS21: spinal cord mantle layer	Present
TS21: spinal cord marginal layer	Present
TS21: stomach mesenchyme	Present
TS21: sympathetic ganglion	Present
TS21: tongue	Present
TS21: vagal nerve trunk	Present
TS21: brain	Present	Single cells		Expression is in scattered cells.
TS21: liver	Present	Single cells		Expression is in scattered cells.
TS21: lung	Present	Single cells		Expression is in scattered cells.
TS21: diencephalon	Present	Regionally restricted		Expression is in a broad band in the dorsal wall and in most of the outer lining.
TS21: telencephalon	Present	Regionally restricted		Expression is in the intermediate zone and the caudate-putamen.
TS21: facial ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS21: trigeminal ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS21: vestibulo-cochlear ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS21: spinal cord	Present	Regionally restricted		Expression is in the neural extensions.
TS21: utricle	Present	Regionally restricted		Expression is in the semicircular canal.
TS21: nasal cavity epithelium	Present	Regionally restricted		Expression is in isolated cells in the most caudal part.
TS21: heart atrium	Present	Single cells		Expression is in scattered cells in the atrium.
TS21: heart	Present	Regionally restricted		Expression is in the dorsal part of the heart sack.
TS21: stomach epithelium	Strong	Regionally restricted		Expression is in a small area where the intestine is diverging.
TS21: intestine	Present	Regionally restricted		Expression is in cells surrounding the edges.
TS21: forelimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS21: hindlimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS21: skeletal muscle	Present	Regionally restricted		Expression is in an area of lung muscle in the neck.
TS21: integumental system	Present	Regionally restricted		Expression is in the primordium of prominent tactile or sinus hair follicles.
TS21: diencephalon lamina terminalis	Absent

Structure	Level	Pattern	Image	Note
TS22: cartilage	Present
TS22: cerebral cortex	Present
TS22: circumvallate papillae	Present
TS22: cornea epithelium	Present
TS22: crus of diaphragm	Present
TS22: esophagus	Present
TS22: lateral ventricle choroid plexus	Present
TS22: lens	Present
TS22: medulla oblongata	Present
TS22: metencephalon part of 4th ventricle choroid plexus	Present
TS22: midbrain	Present
TS22: nasal cavity epithelium	Present
TS22: neural retinal epithelium	Present
TS22: olfactory lobe	Present
TS22: optic nerve	Present
TS22: pigmented retinal epithelium	Present
TS22: pons	Present
TS22: seminiferous cord	Present
TS22: skin	Present
TS22: spinal cord mantle layer	Present
TS22: spinal cord marginal layer	Present
TS22: stomach	Present
TS22: sympathetic ganglion	Present
TS22: thyroid gland	Present
TS22: tongue	Present
TS22: vagal nerve trunk	Present
TS22: brain	Present	Single cells		Expression is in scattered cells.
TS22: liver	Present	Single cells		Expression is in scattered cells.
TS22: skeleton	Present	Regionally restricted		Expression is in the primary center of ossification of all bones.
TS22: diencephalon	Present	Regionally restricted		Expression is in a broad band in the dorsal wall and in most of the outer lining.
TS22: telencephalon	Present	Regionally restricted		Expression is in the intermediate zone and the caudate-putamen.
TS22: spinal cord	Present	Regionally restricted		Expression is in the neural extensions.
TS22: pericardial component	Present	Regionally restricted		Expression is surrounding the pericardial cavity.
TS22: intestine	Present	Regionally restricted		Expression is in cells surrounding the edges.
TS22: forelimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS22: hindlimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS22: skeletal muscle	Present	Regionally restricted		Expression is in an area of lung muscle in the neck.
TS22: integumental system	Present	Regionally restricted		Expression is in the primordium of prominent tactile or sinus hair follicles.
TS22: diencephalon lamina terminalis	Absent

Structure	Level	Pattern	Image	Note
TS23: cartilage	Present
TS23: caudate-putamen	Present
TS23: cerebral cortex	Present
TS23: cornea epithelium	Present
TS23: dorsal root ganglion	Present
TS23: esophagus	Present
TS23: intestine mesenchyme	Present
TS23: medulla oblongata	Present
TS23: midbrain	Present
TS23: nasal cavity epithelium	Present
TS23: neural retina	Present
TS23: olfactory lobe	Present
TS23: optic nerve	Present
TS23: pons	Present
TS23: seminiferous cord	Present
TS23: skin	Present
TS23: spinal cord mantle layer	Present
TS23: spinal cord marginal layer	Present
TS23: stomach mesenchyme	Present
TS23: sympathetic ganglion	Present
TS23: thyroid gland	Present
TS23: tongue	Present
TS23: vagal nerve trunk	Present
TS23: skeleton	Present	Regionally restricted		Expression is in the primary center of ossification of all bones.
TS23: diencephalon	Present	Regionally restricted		Expression is in a broad band in the dorsal wall and in most of the outer lining.
TS23: telencephalon	Present	Regionally restricted		Expression is in the intermediate zone.
TS23: facial ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS23: vestibulo-cochlear ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS23: hypogastric plexus	Present	Regionally restricted		Expression is in the left and right vesicle and inferior hypogastric plexus containing sympathetic and parasympathetic fibers.
TS23: utricle	Present	Regionally restricted		Expression is in the semicircular canal.
TS23: intestine	Present	Regionally restricted		Expression is in cells surrounding the edges.
TS23: testis	Present	Regionally restricted		Expression is in the duct of the seminal vesicle and the ductus deferens.
TS23: forelimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS23: hindlimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS23: integumental system	Present	Regionally restricted		Expression is in the primordium of prominent tactile or sinus hair follicles.
TS23: diencephalon lamina terminalis	Absent

Structure	Level	Pattern	Image	Note
TS24: cartilage	Present
TS24: caudate-putamen	Present
TS24: cerebral cortex	Present
TS24: circumvallate papillae	Present
TS24: cochlear duct epithelium	Present
TS24: dorsal root ganglion	Present
TS24: esophagus	Present
TS24: intestine mesenchyme	Present
TS24: lens	Present
TS24: medulla oblongata	Present
TS24: metanephros	Present
TS24: midbrain	Present
TS24: nasal cavity epithelium	Present
TS24: neural retina	Present
TS24: olfactory lobe	Present
TS24: optic nerve	Present
TS24: ovary	Present
TS24: pons	Present
TS24: skin	Present
TS24: spinal cord mantle layer	Present
TS24: spinal cord marginal layer	Present
TS24: stomach mesenchyme	Present
TS24: sublingual gland	Present
TS24: submandibular gland	Present
TS24: sympathetic ganglion	Present
TS24: tongue	Present
TS24: trigeminal ganglion	Present
TS24: vagal nerve trunk	Present
TS24: skeleton	Present	Regionally restricted		Expression is in the primary center of ossification of all bones.
TS24: diencephalon	Present	Uniform
TS24: telencephalon	Present	Regionally restricted		Expression is in the intermediate zone.
TS24: facial ganglion	Present	Regionally restricted		Expression is in the rootlets.
TS24: hypogastric plexus	Present	Regionally restricted		Expression is in the left and right vesicle and inferior hypogastric plexus containing sympathetic and parasympathetic fibers.
TS24: spinal cord	Present	Regionally restricted		Expression is in the neural extensions.
TS24: utricle	Present	Regionally restricted		Expression is in the semicircular canal.
TS24: forelimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS24: hindlimb	Present	Regionally restricted		Elongated stain in all four limbs.
TS24: hair	Present	Regionally restricted		Expression is in the primordium of prominent tactile or sinus hair follicles.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present		5a

Structure	Level	Pattern	Image	Note
TS17: 3rd ventricle	Present	Regionally restricted	5b	Expression is in dense cell populations as well as scattered cells.
TS17: trunk	Strong	Regionally restricted	5b	Expression is in the lining of the body.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		5c
TS20: head mesenchyme	Present	Single cells	5c

Structure	Level	Pattern	Image	Note
TS21: hypothalamus marginal layer	Strong		5d
TS21: hypothalamus ventricular layer	Present	Regionally restricted	5d

Structure	Level	Pattern	Image	Note
TS20: lateral ventricle	Present	Single cells	5e
TS20: skin	Strong		5e

Structure	Level	Pattern	Image	Note
TS23: caudate-putamen	Strong		5f
TS23: cortical plate	Strong		5f

Structure	Level	Pattern	Image	Note
TS24: caudate-putamen	Present		5g
TS24: cortical plate	Present		5g

Structure	Level	Pattern	Image	Note
TS17: 4th ventricle	Strong	Regionally restricted	5h	Expression is strong in the progenitor zone.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata alar plate mantle layer	Present		5i
TS22: medulla oblongata alar plate marginal layer	Present		5i
TS22: medulla oblongata basal plate mantle layer	Present		5i
TS22: medulla oblongata basal plate marginal layer	Present		5i

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	6a	Expression is in the most dorsal and ventral parts of the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6b	Expression is in the most dorsal and ventral parts of the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6c	Expression is in the most dorsal and ventral parts of the spinal cord.

Structure	Level	Pattern	Image	Note
TS22: spinal cord marginal layer	Present		6d

Structure	Level	Pattern	Image	Note
TS24: spinal cord mantle layer	Present		6e

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted	6f	Expression is in extensions from the spinal cord.
TS24: dorsal root ganglion	Present		6f

Structure	Level	Pattern	Image	Note
TS19: trigeminal ganglion	Present		7a

Structure	Level	Pattern	Image	Note
TS21: esophagus mesenchyme	Present		7b
TS21: sympathetic nervous system	Present		7b
TS21: vagal nerve trunk	Present		7b
TS21: vertebral cartilage condensation	Present		7b

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present		7c
TS19: neural retinal epithelium	Present		7c
TS19: pigmented retinal epithelium	Present		7c

Structure	Level	Pattern	Image	Note
TS21: lens	Present		7d
TS21: neural retinal epithelium	Present		7d
TS21: pigmented retinal epithelium	Present		7d
TS21: retina	Present	Regionally restricted	7d	Expression is in the ciliary body.

Structure	Level	Pattern	Image	Note
TS24: olfactory nerve	Present	Regionally restricted	7e	Expression is in the neuronal projections.

Structure	Level	Pattern	Image	Note
TS22: nose	Present	Regionally restricted	7f	Expression is in the nasal non-neuronal epithelium surrounding the vomeronasal organ.
TS22: vomeronasal organ	Absent		7f	Absent in the sensory neurons.

Structure	Level	Pattern	Image	Note
TS22: gut	Present	Regionally restricted	8a	Expression is in the gastrointestinal wall.

Structure	Level	Pattern	Image	Note
TS24: circumvallate papillae	Present	Regionally restricted	8b	Expression is in the median circumvallate papilla in the dorsal region of the tongue.

Structure	Level	Pattern	Image	Note
TS22: testis	Present		8c

Structure	Level	Pattern	Image	Note
TS24: ovary	Present		8d
TS24: metanephros	Weak	Single cells	8d

Structure	Level	Pattern	Image	Note
TS21: intestine	Present	Regionally restricted	9b , 9e	Postitive cells are localized next to mature smooth muscles.

Structure	Level	Pattern	Image	Note
TS21: intestine	Present	Regionally restricted	9f , 9c	Postitive cells are localized next to mature smooth muscles.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		S2a

Structure	Level	Pattern	Image	Note
TS24: skin	Present		S3a

Structure	Level	Pattern	Image	Note
TS22: diencephalon marginal layer	Present		S3b
TS22: hypothalamus	Present		S3b

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Weak		S3c	Diffuse stain.

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present		S4a , S4b

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present		S4d , S4e

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present		S4h , S4g

Structure	Level	Pattern	Image	Note
TS22: lens	Present		S5a
TS22: pigmented retinal epithelium	Weak		S5a
TS22: cornea epithelium	Strong		S5a
TS22: retina	Strong		S5a	Expression is in the ciliary body.

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present		S5b
TS21: optic nerve	Present		S5b
TS21: pigmented retinal epithelium	Present		S5b

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present		S5c
TS21: pigmented retinal epithelium	Present		S5c

Structure	Level	Pattern	Image	Note
TS23: vibrissa	Present		S5d

Structure	Level	Pattern	Image	Note
TS23: limb	Present	Regionally restricted	S5e	Expression can be observed as projections in all four limbs.