Gene Expression Data
Assay Details
Assay
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Reference:
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J:93589
Kolterud A, et al., The Lim homeobox gene Lhx2 is required for olfactory sensory neuron identity. Development. 2004 Nov;131(21):5319-26
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3623960
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Gene symbol:
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Neurod1
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Gene name:
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neurogenic differentiation 1
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Probe:
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Neurod1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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Control embryos are either heterozygotes or +/+.
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Results
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Specimen
3C Cont:
embryonic day 15.5
(more )
Specimen
3C Cont:
(close )
Genetic Background:
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involves: 129 * 129S4/SvJae * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: olfactory epithelium |
Present |
Regionally restricted |
3C Cont
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Expression is in the one- to two-cell thick layer of progenitor cells.
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Specimen
Not Shown +/-:
embryonic day 15.5; Lhx2tm1Dra/Lhx2 +
(more )
Specimen
Not Shown +/-:
(close )
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2+
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Age:
|
embryonic day 15.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: olfactory epithelium |
Present |
Regionally restricted |
|
Expression is in the one- to two-cell thick layer of progenitor cells.
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Specimen
3C Lhx2-/-:
(close )
Genetic Background:
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involves: 129 * 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2tm1Dra
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Age:
|
embryonic day 15.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
Not Shown, +/+ Control:
embryonic day 14.5
(more )
Specimen
Not Shown, +/+ Control:
(close )
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: olfactory epithelium |
Present |
Regionally restricted |
|
Expression is in the one- to two-cell thick layer of progenitor cells.
|
Specimen
Not Shown, +/- Control:
embryonic day 14.5; Lhx2tm1Dra/Lhx2 +
(more )
Specimen
Not Shown, +/- Control:
(close )
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6
|
Mutant Allele(s):
|
Lhx2tm1Dra/Lhx2+
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: olfactory epithelium |
Present |
Regionally restricted |
|
Expression is in the one- to two-cell thick layer of progenitor cells.
|
Specimen
Not Shown, -/-:
(close )
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6
|
Mutant Allele(s):
|
Lhx2tm1Dra/Lhx2tm1Dra
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Age:
|
embryonic day 14.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: olfactory epithelium |
Present |
|
|
Positive cells have expanded into the presumptive neuronal cell layer.
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