Probe:	Ao probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: liver	Weak

Structure	Level	Pattern	Image	Note
TS22: liver	Strong		2A
TS22: cartilage	Present		2A
TS22: foregut mesenchyme	Present	Regionally restricted	2A	Expression was detected in the submucosal mesenchyme of the foregut.
TS22: heart	Absent		2A
TS22: lung	Absent		2A
TS22: metanephros	Absent		2A

Structure	Level	Pattern	Image	Note
TS25: skin	Present	Regionally restricted		Expression was detected in the subepidermal layer of the skin.
TS25: heart	Absent
TS25: lung	Absent

Structure	Level	Pattern	Image	Note
TS25: medullary tubule	Present		4a	Expression was detected in the proximal tubules.
TS25: glomerular tuft	Absent		4a
TS25: early nephron	Absent		4a	Expression was absent from the proximal tubules of the cortex, the comma or S-shaped bodies, the collecting tubules and the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS27: outer stripe of outer medulla	Present	Regionally restricted		Expression was detected in the proximal tubules in the medulla (i.e. proximal straight tubules).
TS27: renal proximal tubule	Absent			Expression was absent from the proximal tubules in the cortex.
TS27: glomerular tuft	Absent			Expression was absent from the glomeruli, comma or S-shaped bodies, collecting tubules and mesenchymal cells.
TS27: renal corpuscle	Absent			Expression was absent from the glomeruli, comma or S-shaped bodies, collecting tubules and mesenchymal cells.
TS27: collecting duct	Absent			Expression was absent from the glomeruli, comma or S-shaped bodies, collecting tubules and mesenchymal cells.
TS27: renal interstitium	Absent			Expression was absent from the glomeruli, comma or S-shaped bodies, collecting tubules and mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS28: outer stripe of outer medulla	Strong		4c
TS28: inner stripe of outer medulla	Present	Patchy	4c	Scattered signals were detected in the inner stripe of outer medulla along the descending limb of Henle's loop.
TS28: proximal straight tubule, cortical portion	Strong	Regionally restricted	4c	Expression was detected in the medullary ray.

Structure	Level	Pattern	Image	Note
TS28: outer stripe of outer medulla	Strong		4e
TS28: inner stripe of outer medulla	Present	Patchy	4e	Expression was detected in the inner stripe of outer medulla along the descending limb of Henle's loop.
TS28: proximal straight tubule, cortical portion	Strong	Regionally restricted	4e	Expression was detected in the medullary ray.