Probe:	Ebfaz cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS9: embryo	Present			Expression is present throughout the epiblast.
TS9: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS11: allantois mesoderm	Present		4b
TS11: embryo ectoderm	Present	Regionally restricted	4b	Expression is present in the posterior embryonic ectoderm.
TS11: future spinal cord neural plate	Present	Regionally restricted	4b	Expression is present in the anterior and lateral boundaries of the future neural plate.
TS11: embryo mesoderm	Present	Regionally restricted	4b	Expression is present in the posterior embryonic mesoderm as it exits the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Strong		4c	Expression is present throughout the neural plate and tube.
TS13: future midbrain	Strong		4c	Expression is present throughout the neural plate and tube.
TS13: future prosencephalon	Strong		4c	Expression is present throughout the neural plate and tube.
TS13: future spinal cord	Strong		4c	Expression is present throughout the neural plate and tube.
TS13: embryo ectoderm	Present	Regionally restricted	4c	Expression is present in the posterior streak ectoderm.
TS13: paraxial mesenchyme	Present		4c
TS13: somite	Present		4c
TS13: extraembryonic component	Present	Regionally restricted	4c	Expression is present in the allantois.
TS13: heart	Absent		4c
TS13: yolk sac	Absent		4c

Structure	Level	Pattern	Image	Note
TS15: future brain	Present
TS15: future spinal cord	Present
TS15: tail	Present
TS15: trunk somite	Present
TS15: head	Present			Expression is present in craniofacial primordia.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		4d	Expression patterns in tissues is similar between E9.5-E12.5 with additional sites of expression in the developing limbs.
TS17: brain	Present		4d
TS17: future spinal cord	Present		4d
TS17: limb	Present		4d
TS17: tail	Present		4d
TS17: trunk somite	Present		4d

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		4e	Expression patterns in tissues is similar between E9.5-E12.5 with additional sites of expression in the developing limbs.
TS20: brain	Present		4e
TS20: limb	Present		4e
TS20: spinal cord	Present		4e
TS20: tail	Present		4e

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present		4f	Expression is present in the whisker barrels.
TS20: telencephalon	Present	Regionally restricted	4f	Expression is present in the olfactory bulb progenitors.