Tg(Ins2-GAD2)2Lt
Transgene Detail
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| Symbol: |
Tg(Ins2-GAD2)2Lt |
| Name: |
transgene insertion 2, Edward H Leiter |
| MGI ID: |
MGI:3603349 |
| Synonyms: |
TgN(RIP7hGAD65)Lt4 (A line), TgN(RIP7hGAD65)Lt4 A-line |
| Transgene: |
Tg(Ins2-GAD2)2Lt Location: unknown Genetic Position: Chr15, Syntenic
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| Alliance: |
Tg(Ins2-GAD2)2Lt page
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| Transgene Type: |
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Transgenic (Humanized sequence, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Ins2-GAD2)2Lt expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
GAD2 (2572) |
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Tg(Ins2-GAD2)2Lt expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| rat |
Ins2 (24506) |
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Mutation details: The transgene comprises the cDNA sequence encoding human glutamic acid decarboxylase 2 under control of a modified rat insulin 2 promoter composed of 9.5 kb of genomic DNA from upstream of the transcription initiation site fused to the first intron of Ins2. Densitrometric Southern blot analysis indicates presence of 4-5 copies of the transgene. FISH analysis and pseudo-Giemsa metaphase spread analysis showed integration to have occurred near the centromere of Chr 15. Expression in the pancreatic islets of transgenic mRNA has been demonstrated by reverse transcription followed by PCR (RT-PCR), and of GAD2 protein by western blot analysis.
(J:100251)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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| Original: |
J:100251 Bridgett M, et al., Differential protection in two transgenic lines of NOD/Lt mice hyperexpressing the autoantigen GAD65 in pancreatic beta-cells. Diabetes. 1998 Dec;47(12):1848-56 |
| All: |
1 reference(s) |
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Analysis Tools
