Gene Expression Data
Assay Details
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Assay
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Reference:
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J:97538
Pelka GJ, et al., Distinct expression profiles of Mecp2 transcripts with different lengths of 3'UTR in the brain and visceral organs during mouse development. Genomics. 2005 Apr;85(4):441-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3590654
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Gene symbol:
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Mecp2
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Gene name:
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methyl CpG binding protein 2
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Probe:
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pGP1710
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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All time points were assayed using both probes, pGP1710 (MGI:3590558) and pGP14all (MGI:3590555); both probes showed similar results. Only results shown in Figures or included in Table 1 are shown below.
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Results
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Specimen
1D:
embryonic day 6.5
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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129X1/SvJ
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Age:
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embryonic day 6.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1H:
embryonic day 12.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1R:
postnatal week 20
(more )
Specimen
1R:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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postnatal week 20
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1W:
postnatal week 20
(more )
Specimen
1W:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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postnatal week 20
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Table 1 E10.5:
embryonic day 10.5
(more )
Specimen
Table 1 E10.5:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Strong |
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Expression is stated as being strong to moderate. Expression is stated as being in skin and was coded to surface ectoderm.
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| TS17: gut |
Strong |
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Expression is stated as being strong to moderate. Expression is stated as being in skin and was coded to surface ectoderm.
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| TS17: heart |
Strong |
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Expression is stated as being strong to moderate. Expression is stated as being in skin and was coded to surface ectoderm.
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| TS17: hindlimb bud |
Strong |
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Expression is stated as being strong to moderate. Expression is stated as being in skin and was coded to surface ectoderm.
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| TS17: midbrain |
Strong |
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Expression is stated as being strong to moderate. Expression is stated as being in skin and was coded to surface ectoderm.
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| TS17: surface ectoderm |
Strong |
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Expression is stated as being strong to moderate. Expression is stated as being in skin and was coded to surface ectoderm.
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| TS17: telencephalon |
Strong |
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Expression is stated as being strong to moderate. Expression is stated as being in skin and was coded to surface ectoderm.
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| TS17: hindbrain |
Strong |
Regionally restricted |
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Expression is stated as being strong to moderate. Expression was detected in the myelencephalon.
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Specimen
Table 1 E12.5:
embryonic day 12.5
(more )
Specimen
Table 1 E12.5:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Table 1 E14.5:
embryonic day 14.5
(more )
Specimen
Table 1 E14.5:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Table 1 E16.5:
embryonic day 16.5
(more )
Specimen
Table 1 E16.5:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2O:
postnatal week 11
(more )
Specimen
2O:
(close )
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Genetic Background:
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129X1/SvJ
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Age:
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postnatal week 11
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools