About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:3969 Zhou X, et al., Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation. Nature. 1993 Feb 11;361(6412):543-7
Assay type: RNA in situ
MGI Accession ID: MGI:3589355
Gene symbol: Nodal
Gene name: nodal growth differentiation factor
Probe: Nodal cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4a left: embryonic day 7.5 (more )
Note: This embryo is at head fold stage with no visible somites. This view is of the ventral surface with the posterior end up.
Structure Level Pattern Image Note
TS11: node Present 4a left Expression is seen as a ring of staining around the node.
TS11: primitive streak Present Regionally restricted 4a left No expression in the posterior primitive streak
TS11: neural ectoderm Absent 4a left No expression in the neural folds at the anterior end of the embryo.
TS11: allantois Absent 4a left
Specimen 4a middle: embryonic day 7.5 (more )
Note: This embryo is at head fold stage with no visible somites.
Structure Level Pattern Image Note
TS11: node Present 4a middle Expression is seen as a ring of staining around the node.
TS11: allantois Absent 4a middle
Specimen 4a right - left embryo: embryonic day 7.0 (more )
Note: This embryo is at later primitive streak stage but contains a node.
Structure Level Pattern Image Note
TS10: node Present 4a right - left embryo
Specimen 4a right - right embryo: embryonic day 7.0 (more )
Note: This embryo is at later primitive streak stage but has no node.
Structure Level Pattern Image Note
TS10: embryo Weak 4a right - right embryo There is a very low level of expression and no discrete node.
Specimen 4b left: embryonic day 8.0 (more )
Note: This embryo is at head fold stage. This is a parasagittal section.
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4b left Expression is present in the node in a small group of external cells (possibly endoderm) and cells immediately subadjacent to them.
Specimen 4b right: embryonic day 8.0 (more )
Note: This embryo is at head fold stage. This is a parasagittal section.
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4b right Expression is present in the node in a small group of external cells (possibly endoderm) and cells immediately subadjacent to them.
Specimen Not shown: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS12: embryo Absent Lack of expression coincides with disappearance of the node as a distinct structure.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory