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Dicer1tm1Dli
Targeted Allele Detail
Summary
Symbol: Dicer1tm1Dli
Name: dicer 1, ribonuclease type III; targeted mutation 1, David M Livingston
MGI ID: MGI:3589287
Synonyms: dcrfl
Gene: Dicer1  Location: Chr12:104654001-104718211 bp, - strand  Genetic Position: Chr12, 54.83 cM, cytoband E-F1
Alliance: Dicer1tm1Dli page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:96031
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsLoxP sites were inserted to flank exons 18-20, which encode part of the first RNase III domain. Deletion of this sequence putatively leads to out-of-frame splicing of all downstream exons, including the second RNase III and the dsRNA-binding domains, and results in complete ablation of enzymactic activity. An frt-flanked neo was inserted into intron 17 and was removed by crossing mice with a strain that expresses Flp recombinase in the germ line. (J:100546)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Dicer1 Mutation:  94 strains or lines available
References
Original:  J:96031 Kanellopoulou C, et al., Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. Genes Dev. 2005 Feb 15;19(4):489-501
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory