Phenotypes associated with this allele

• Allele Symbol: Dicer1^tm1Dli
• Allele Name: targeted mutation 1, David M Livingston
• Allele ID: MGI:3589287
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Dicer1^tm1Dli/Dicer1^tm1Dli Tg(Cd4-cre)1Cwi/?	involves: 129 * C57BL/6 * DBA/2	MGI:3590208

Genotype
MGI:3590208

cn1

• Allelic Composition: Dicer1^tm1Dli/Dicer1^tm1Dli; Tg(Cd4-cre)1Cwi/?
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell physiology ( J:100546 )

• decreased mature miR-150 in thymocytes, naive peripheral CD4+ T cells and Th2 cells with increased pre-miR-150 found in naive peripheral CD4+ and Th2 cells

• decreased mature miR-21 in thymocytes, Th1 and Th2 cells with increased pre-miR-21 found in Th1 and Th2

• decreased mature miR-103 and miR-29 in T cells but with no accumulation of their precursor polypeptides

increased T cell apoptosis ( J:100546 )

• stimulation of Th1 or Th2 cells in culture causes increased apoptosis

decreased T cell proliferation ( J:100546 )

• stimulation of Th1 or Th2 cells in culture yields slower proliferation than normal, decreased expansion and increased apoptotic and dead cells

immune system

immune system phenotype ( J:100546 )

N • normal thymocyte number and subpopulations

increased T cell apoptosis ( J:100546 )

• stimulation of Th1 or Th2 cells in culture causes increased apoptosis

decreased T cell proliferation ( J:100546 )

• stimulation of Th1 or Th2 cells in culture yields slower proliferation than normal, decreased expansion and increased apoptotic and dead cells

decreased T cell number ( J:100546 )

• spleen and lymph nodes have fewer CD3+ cells with a decreased percentage (fourfold reduction in spleen) of CD8+ T cells and a smaller decrease (twofold reduction) in percentage of CD4+ T cells

abnormal T-helper 1 physiology ( J:100546 )

• ThN cultures have increased IFNG expression leading to Th1 production

• cultured Th1 cells show accelerated loss of stimulation-induced IL2 expression

abnormal T-helper 2 physiology ( J:100546 )

• Th2 cultures have 30% lower expression of Gata3 transcripts

• IFNG expression is not appropriately repressed in Th2 cultures

abnormal cytotoxic T cell physiology ( J:100546 )

hematopoietic system

increased T cell apoptosis ( J:100546 )

• stimulation of Th1 or Th2 cells in culture causes increased apoptosis

decreased T cell proliferation ( J:100546 )

• stimulation of Th1 or Th2 cells in culture yields slower proliferation than normal, decreased expansion and increased apoptotic and dead cells

decreased T cell number ( J:100546 )

• spleen and lymph nodes have fewer CD3+ cells with a decreased percentage (fourfold reduction in spleen) of CD8+ T cells and a smaller decrease (twofold reduction) in percentage of CD4+ T cells

abnormal T-helper 1 physiology ( J:100546 )

• ThN cultures have increased IFNG expression leading to Th1 production

• cultured Th1 cells show accelerated loss of stimulation-induced IL2 expression

abnormal T-helper 2 physiology ( J:100546 )

• Th2 cultures have 30% lower expression of Gata3 transcripts

• IFNG expression is not appropriately repressed in Th2 cultures

abnormal cytotoxic T cell physiology ( J:100546 )