Probe:	EMAP II cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present	Regionally restricted		Expression is dorsal.
TS13: future brain	Present	Regionally restricted		Expression is dorsal.
TS13: neural tube	Present	Regionally restricted		Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch	Present	Regionally restricted		Expression is dorsal.
TS14: future brain	Present	Regionally restricted		Expression is dorsal.
TS14: neural tube	Present	Regionally restricted		Expression is dorsal.
TS14: cardiovascular system	Absent
TS14: limb	Absent
TS14: embryo mesenchyme	Absent			Absent in the skeleton.

Structure	Level	Pattern	Image	Note
TS18: 1st branchial arch	Present		1
TS18: 2nd branchial arch	Present		1
TS18: 3rd branchial arch	Present		1
TS18: 4th ventricle	Present		1
TS18: cerebellum primordium	Present		1
TS18: dorsal root ganglion	Present		1
TS18: heart	Present		1
TS18: lateral ventricle	Present		1
TS18: mesencephalic vesicle	Present		1
TS18: neural tube	Present		1
TS18: olfactory epithelium	Present		1
TS18: otocyst	Present		1
TS18: eye	Present	Regionally restricted	1	Expression is in the optic cusp.
TS18: intestine	Present	Regionally restricted	1	Expression is both in the epithelial cells and at the epithelial-mesenchymal interface.
TS18: stomach epithelium	Present	Regionally restricted	1	Expression is both in the epithelial cells and at the epithelial-mesenchymal interface.
TS18: stomach mesenchyme	Present	Regionally restricted	1	Expression is both in the epithelial cells and at the epithelial-mesenchymal interface.
TS18: liver	Moderate	Regionally restricted	1	Expression is regions consistent with the hepatic sinuses.
TS18: urogenital sinus	Moderate	Regionally restricted	1	Expression is in the epithelial cells and at the epithelial-mesenchymal junction.

Structure	Level	Pattern	Image	Note
TS22: heart	Strong	Regionally restricted	2A	Expression is in the septal region of the heart.
TS22: atrium cardiac muscle	Present		2A
TS22: ventricle cardiac muscle	Present		2A

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		2B

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Strong	Regionally restricted	2C	Expression is in the stroma and is absent in the endothelium.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Strong	Regionally restricted	2D	Expression is at the epithelial-mesenchymal junction.

Structure	Level	Pattern	Image	Note
TS22: urogenital sinus	Strong	Regionally restricted	2E	Expression is at the epithelial-mesenchymal junction.

Structure	Level	Pattern	Image	Note
TS22: intestine	Very strong	Regionally restricted	2F	Expression is at the epithelial-mesenchymal junction.

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Strong	Regionally restricted	2G	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present	Regionally restricted	2H

Structure	Level	Pattern	Image	Note
TS21: brain mantle layer	Present
TS21: cochlear duct epithelium	Present
TS21: neural retinal epithelium	Present
TS21: pharyngo-tympanic tube	Present
TS21: spinal cord mantle layer	Present
TS21: spinal cord roof plate	Present
TS21: superior cervical ganglion	Present
TS21: brain	Present	Regionally restricted		Expression is in the roof plate.
TS21: nervous system	Present	Regionally restricted		Expression is in the cranial epithelium and nerves.

Structure	Level	Pattern	Image	Note
TS22: diencephalon lamina terminalis	Strong
TS22: hindbrain	Strong
TS22: pons	Strong
TS22: trigeminal ganglion	Strong
TS22: nervous system	Strong	Regionally restricted		Expression is in the cranial epithelium and nerves.
TS22: brain meninges	Present
TS22: midbrain	Present
TS22: limb	Absent
TS22: skeleton	Absent

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Moderate
TS22: olfactory epithelium	Moderate
TS22: nasal cavity	Present	Regionally restricted		Absent in the mesenchyme surrounding the olfactory epithelium.
TS22: neural retinal epithelium	Moderate
TS22: lung epithelium	Strong			Strong in the epithelial/mesenchymal junction, weak in the rest of the epithelium and mesenchyme.
TS22: lung mesenchyme	Strong			Strong in the epithelial/mesenchymal junction, weak in the rest of the epithelium and mesenchyme.
TS22: esophagus epithelium	Moderate
TS22: esophagus mesenchyme	Moderate
TS22: intestine epithelium	Strong	Regionally restricted
TS22: intestine mesenchyme	Absent
TS22: metanephros	Moderate	Regionally restricted		Expression is in the epithelial/mesenchymal junction, and in the rest of the epithelium, absent in the mesenchyme.
TS22: mesenchyme of metanephros	Absent
TS22: urogenital sinus	Moderate	Regionally restricted		Expression is in the epithelial/mesenchymal junction, and in the rest of the epithelium, absent in the mesenchyme.
TS22: bladder	Moderate	Regionally restricted		Expression is in the epithelium absent in the mesenchyme.
TS22: heart	Moderate
TS22: dorsal aorta	Moderate	Regionally restricted		Expression is in the stroma, absent in the endothelial cells.
TS22: liver	Very strong
TS22: choroid plexus	Very strong
TS22: dorsal root ganglion	Very strong
TS22: spinal cord	Very strong
TS22: sympathetic ganglion	Very strong
TS22: trigeminal ganglion	Very strong
TS22: midbrain	Moderate
TS22: cartilage	Absent
TS22: cerebral cortex	Absent
TS22: skeletal muscle	Absent

Structure	Level	Pattern	Image	Note
TS24: vibrissa	Very strong	Regionally restricted		Expression is at the epithelial-mesenchymal junction, and is absent in the surrounding mesenchymal cells.
TS24: skin	Present			More prominent on the face and limb.
TS24: inner ear epithelium	Present
TS24: olfactory epithelium	Present
TS24: submandibular gland	Present
TS24: tongue epithelium	Present	Regionally restricted		Expression is on the dorsal surface.
TS24: liver	Weak
TS24: tongue muscle	Weak
TS24: inner ear mesenchyme	Absent
TS24: skeleton	Absent