Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:97065
Asayesh A, et al., Developmental expression of metalloproteases ADAM 9, 10, and 17 becomes restricted to divergent pancreatic compartments. Dev Dyn. 2005 Apr;232(4):1105-14
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3580821
|
Gene symbol:
|
Gcg
|
Gene name:
|
glucagon
|
Antibody:
|
Anti-Glucagon
|
Detection system:
|
Secondary antibody
|
Assay notes:
|
The images were confocal images.
|
|
Results
|
Specimen
1F, 1I:
embryonic day 10.0
(more )
Note: Double labeled: green - Gcg; red - Adam9 (assay MGI:3580815).
Specimen
1F, 1I:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam9 (assay MGI:3580815).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: pancreas primordium |
Present |
Regionally restricted |
1F
,
1I
|
Expression was detected at the pancreatic anterior-posterior level. Expression overlaps with Adam9.
|
Specimen
1G, 1J:
embryonic day 10.0
(more )
Note: Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
Specimen
1G, 1J:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: pancreas primordium |
Present |
Regionally restricted |
1G
,
1J
|
Expression was detected at the pancreatic anterior-posterior level. Expression overlapped with Adam10 at low or nearly absent levels.
|
Specimen
1H, 1K:
embryonic day 10.0
(more )
Note: Double labeled: green - Gcg; red - Adam17 (assay MGI:3580820).
Specimen
1H, 1K:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam17 (assay MGI:3580820).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: pancreas primordium |
Present |
Regionally restricted |
1K
,
1H
|
Expression was detected at the pancreatic anterior-posterior level. Expression overlaps with Adam17.
|
Specimen
2J:
embryonic day 12.5
(more )
Note: Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
Specimen
2J:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Present |
Regionally restricted |
2J
|
Expression was detected in the endocrine compartment.
|
Specimen
2K:
embryonic day 15.5
(more )
Note: Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
Specimen
2K:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
Regionally restricted |
2K
|
Expression was detected in the endocrine compartment.
|
Specimen
2L:
embryonic day 17.5
(more )
Note: Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
Specimen
2L:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: pancreas |
Present |
Regionally restricted |
2L
|
Expression was detected in the endocrine compartment.
|
Specimen
3B:
postnatal day 1
(more )
Note: Double labeled: green - Gcg; red - Adam9 (assay MGI:3580815).
Specimen
3B:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam9 (assay MGI:3580815).
|
Specimen
3E:
postnatal day 1
(more )
Note: Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
Specimen
3E:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam10 (assay MGI:3580818).
|
Specimen
3H:
postnatal day 1
(more )
Note: Double labeled: green - Gcg; red - Adam17 (assay MGI:3580820).
Specimen
3H:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam17 (assay MGI:3580820).
|
Specimen
4B:
postnatal week 8
(more )
Note: Double labeled: green - Gcg; red - Adam9 (assay MGI:3580815).
Specimen
4B:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal week 8
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam9 (assay MGI:3580815).
|
Specimen
4E:
postnatal week 8
(more )
Note: Double labeled: green - Gcg; red - Adam17 (assay MGI:3580820).
Specimen
4E:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal week 8
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Gcg; red - Adam17 (assay MGI:3580820).
|
|