Gene Expression Data
Assay Details
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Assay
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Reference:
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J:97065
Asayesh A, et al., Developmental expression of metalloproteases ADAM 9, 10, and 17 becomes restricted to divergent pancreatic compartments. Dev Dyn. 2005 Apr;232(4):1105-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3580820
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Gene symbol:
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Adam17
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Gene name:
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a disintegrin and metallopeptidase domain 17
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Antibody:
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anti-ADAM17
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Detection system:
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Secondary antibody
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Assay notes:
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The images were confocal images.
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Results
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Specimen
1E, 1K:
embryonic day 10.0
(more  )
Note: Double labeled: red - Adam17; green - Gcg (assay MGI:3580821).
Specimen
1E, 1K:
(close  )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Gcg (assay MGI:3580821).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: pancreas primordium |
Present |
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1K
,
1E
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Expression was detected in both the dorsal and ventral pancreatic buds, as well as in the intervening gut endoderm. Expression was detected in the majority of the pancreatic primordial cells, including the early glucagon-producing cells.
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Specimen
Table 1:
embryonic day 9.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: pancreas primordium |
Present |
Regionally restricted |
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Expression is present in the pancreatic epithelium as well as in pancreas-associated mesenchyme.
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Specimen
2P:
embryonic day 12.5
(more )
Specimen
2P:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Present |
Uniform |
2P
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Expression was uniformly distributed throughout the pancreas corresponding to both the Ptf1a-positive (exocrine) and Isl1-positive (endocrine) subpopulations.
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Specimen
2Q:
embryonic day 15.5
(more )
Specimen
2Q:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Uniform |
2Q
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Expression was uniformly distributed throughout the pancreas corresponding to both the Ptf1a-positive (exocrine) and Isl1-positive (endocrine) subpopulations.
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Specimen
2R:
embryonic day 17.5
(more )
Specimen
2R:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pancreas |
Present |
Non-Uniform |
2R
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Expression was reduced in the exocrine compartment relative to the endocrine compartment.
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Specimen
Table 1:
embryonic day 14.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pancreas |
Present |
Uniform |
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Expression was uniformly distributed throughout the pancreas.
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Specimen
Table 1:
embryonic day 16.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: pancreas |
Present |
Uniform |
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Expression was uniformly distributed throughout the pancreas.
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Specimen
3G:
postnatal day 1
(more )
Note: Double labeled: red - Adam17; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
3G:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
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Specimen
3H:
postnatal day 1
(more )
Note: Double labeled: red - Adam17; green - Gcg (assay MGI:3580821).
Specimen
3H:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Gcg (assay MGI:3580821).
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Specimen
3I:
postnatal day 1
(more )
Note: Double labeled: red - Adam17; green - Sst (assay MGI:3580819).
Specimen
3I:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Sst (assay MGI:3580819).
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Specimen
4D:
postnatal week 8
(more )
Note: Double labeled: red - Adam17; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Present |
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4D
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Expression was detected in all three endocrine subpopulations investigated.
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Specimen
4E:
postnatal week 8
(more )
Note: Double labeled: red - Adam17; green - Gcg (assay MGI:3580821).
Specimen
4E:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Gcg (assay MGI:3580821).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Present |
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4E
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Expression was detected in all three endocrine subpopulations investigated.
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Specimen
4F:
postnatal week 8
(more )
Note: Double labeled: red - Adam17; green - Sst (assay MGI:3580819).
Specimen
4F:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Sst (assay MGI:3580819).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Present |
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4F
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Expression was detected in all three endocrine subpopulations investigated.
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Specimen
5G, 5I:
postnatal adult
(more )
Note: Double labeled: red - Adam17; green - Cdh1 (assay MGI:3580832).
Specimen
5G, 5I:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Adam17; green - Cdh1 (assay MGI:3580832).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
Present |
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5G
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5I
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Expression was detected in the cell cytoplasm but was not detectable in the outer cell membrane of the islets. Expression did not colocalize with Cdh1 (E-Cadherin).
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Specimen
6G, 6I:
embryonic day 15.5
(more )
Note: This specimen was also stained for DBA-lectin (green).
Specimen
6G, 6I:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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This specimen was also stained for DBA-lectin (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Regionally restricted |
6I
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6G
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Expression was detected in pancreatic ductal cells.
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Specimen
6P, 6R:
postnatal week 8
(more )
Note: This specimen was also stained for DBA-lectin (green).
Specimen
6P, 6R:
(close )
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Genetic Background:
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involves: C57BL * CBA
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Age:
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postnatal week 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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This specimen was also stained for DBA-lectin (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreatic duct |
Present |
Regionally restricted |
6P
,
6R
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Expression was detected in the ductal epithelium.
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