Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:85573
Williams SE, et al., Ephrin-B2 and EphB1 mediate retinal axon divergence at the optic chiasm. Neuron. 2003 Sep 11;39(6):919-35
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3578629
|
|
Gene symbol:
|
Ephb1
|
|
Gene name:
|
Eph receptor B1
|
|
Probe:
|
EphB1 cDNA3
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Fluorescein
|
|
Assay notes:
|
The probe used in this assay recognizes the extracellular domain. Alternatively, it is possible a probe to the 3' end of the transcript was used in this assay (MGI:3578308). The probe was detected with an anti-digoxigenin antibody using a TSA-fluorescein fluorescent substrate system.
|
|
|
Results
|
Specimen
lacZ/+:
embryonic day 17.0; Ephb1tm2Cmn/Ephb1 +
(more  )
Note: This is a frontal section.
Specimen
lacZ/+:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Ephb1tm2Cmn/Ephb1+
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Note:
|
This is a frontal section.
|
Specimen
lacZ/-:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Ephb1tm1Cmn/Ephb1tm2Cmn
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Note:
|
This is a frontal section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: retina |
Absent |
|
S2Bii
|
|
Specimen
lacZ/+:
embryonic day 17.0; Ephb1tm2Cmn/Ephb1 +
(more )
Note: This is a frontal section.
Specimen
lacZ/+:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Ephb1tm2Cmn/Ephb1+
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Note:
|
This is a frontal section.
|
Specimen
lacZ/-:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Ephb1tm1Cmn/Ephb1tm2Cmn
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Note:
|
This is a frontal section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: brain |
Absent |
|
S2Biv
|
|
|
Analysis Tools