Probe:	EphB1 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The probe used in this assay recognizes the extracellular domain. Alternatively, it is possible a probe to the 3' end of the transcript was used in this assay (MGI:3578308).

Structure	Level	Pattern	Image	Note
TS20: retina	Absent		5A	There is little detectable expression in the retina.

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	5B	Expression is present centrally, in both temporal and nasal retina.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	5C	Expression is present in the dorsocentral retina.

Structure	Level	Pattern	Image	Note
TS23: retina	Strong	Regionally restricted	5D	There is strong expression in the ventrotemporal retina, but there is no expression in the dorsocentral retina.

Structure	Level	Pattern	Image	Note
TS25: retina	Present		5E	Expression in the ventrotemporal retina is signficantly reduced from that seen earlier, but it is upregulated elsewhere in the retina.
TS25: retina inner nuclear layer	Strong	Regionally restricted	5E	The strongest expression is confined to a small number of retinal ganglion cells bordering the lens. Expression is also found in dorsal and nasal ganglion cells at this age.
TS25: retina outer nuclear layer	Present		5E

Structure	Level	Pattern	Image	Note
TS20: retina	Absent		5F	There is little detectable expression in the retina.

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present	Regionally restricted	5G	Expression is present in the dorsocentral retina in the retinal ganglion cells layer. It is absent in the ventrotemporal retina.

Structure	Level	Pattern	Image	Note
TS22: retina	Present		5H	Expression is present in the ventrotemporal and dorsocentral retina.

Structure	Level	Pattern	Image	Note
TS23: retina	Strong	Regionally restricted	5I	There is strong expression in the ventrotemporal retina, but there is no expression in the dorsocentral retina.

Structure	Level	Pattern	Image	Note
TS25: retina	Present		5J	Expression in the ventrotemporal retina is signficantly reduced from that seen earlier, but it is upregulated elsewhere in the retina.
TS25: retina inner nuclear layer	Strong	Regionally restricted	5J	The strongest expression is confined to a small number of retinal ganglion cells bordering the lens. Expression is also found in dorsal and nasal ganglion cells at this age.
TS25: retina outer nuclear layer	Present		5J

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	S1F	Expression is present in a small subset of retinal ganglion cells in the ventrotemporal retina.