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Gene Expression Data
Assay Details
Assay
Reference: J:85573 Williams SE, et al., Ephrin-B2 and EphB1 mediate retinal axon divergence at the optic chiasm. Neuron. 2003 Sep 11;39(6):919-35
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:3578406
Gene symbol: Efnb2
Gene name: ephrin B2
Reporter: lacZ
Antibody: anti-B-galactosidase (Biogenesis, 4600-1409)
Assay notes: It is possible that another B-galactosidase antibody was used in this assay (MGI:3578332).
Results
Specimen 2D: embryonic day 15.5; Efnb2tm1Nwg/Efnb2+ (more )
Note: This is a frontal section from the anterior chiasm.
Structure Level Pattern Image Note
TS23: diencephalon Present 2D Expression is broadly located around the floor of the third ventricle.
TS23: optic chiasma Present 2D Expression is broadly located around the floor of the third ventricle.
Specimen 2E: embryonic day 15.5; Efnb2tm1Nwg/Efnb2+ (more )
Note: This is a frontal section from the midchiasm.
Structure Level Pattern Image Note
TS23: optic chiasma Present 2E Expression is strongest in the region in which retinal axons pause and turn to project ipsilaterally.
Specimen 2F: embryonic day 15.5; Efnb2tm1Nwg/Efnb2+ (more )
Note: This is a frontal section from the posterior chiasm.
Structure Level Pattern Image Note
TS23: optic chiasma Present 2F In more caudal sections, staining diminishes.
Specimen 2G: embryonic day 15.5; Efnb2tm1Nwg/Efnb2+ (more )
Note: This is a frontal section from the midchiasm.
Structure Level Pattern Image Note
TS23: optic chiasma Present 2I , 2G Double staining experiments with cell-type specific markers suggest that the expressing cells are glia.
TS23: blood vessel Strong 2G
Specimen 2J: embryonic day 15.5; Efnb2tm1Nwg/Efnb2+ (more )
Note: This is a frontal section from the midchiasm.
Structure Level Pattern Image Note
TS23: optic chiasma Present 2J , 2M Double staining experiments with cell-type specific markers suggest that the expressing cells are glia.
TS23: blood vessel Strong 2J
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory