Gene Expression Data
Assay Details
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Assay
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Reference:
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J:59925
Miner JH, et al., Defective glomerulogenesis in the absence of laminin alpha5 demonstrates a developmental role for the kidney glomerular basement membrane. Dev Biol. 2000 Jan 15;217(2):278-89
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3528026
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Gene symbol:
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Lamb1
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Gene name:
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laminin B1
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Antibody:
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mAb 5A2
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Detection system:
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Secondary antibody
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Assay notes:
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Embryos were not fixed prior to sectioning. Authors state that sections were then sometimes fixed in acetone, ethanol or 2% paraformaldehyde.
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Results
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Specimen
not shown:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Lama5tm1Jhm/Lama5tm1Jhm
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: nephric duct |
Present |
Regionally restricted |
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Authors report that expression colocalized with laminin [a]5; expression in basement membranes associated with the Wollfian duct and recently branched ureteric bud.
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| TS18: ureteric bud |
Present |
Regionally restricted |
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Authors report that expression colocalized with laminin [a]5; expression in basement membranes associated with the Wollfian duct and recently branched ureteric bud.
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Specimen
6F:
embryonic day 16.5
(more  )
Specimen
6F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: collecting duct |
Present |
Regionally restricted |
6F
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Expression in both tubular and collecting duct basement membranes in the medulla.
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| TS24: medullary renal tubule |
Present |
Regionally restricted |
6F
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Expression in both tubular and collecting duct basement membranes in the medulla.
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Lama5tm1Jhm/Lama5tm1Jhm
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: collecting duct |
Present |
Regionally restricted |
6H
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Expression in both tubular and collecting duct basement membranes in the medulla.
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| TS24: medullary renal tubule |
Present |
Regionally restricted |
6H
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Expression in both tubular and collecting duct basement membranes in the medulla.
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Analysis Tools