Probe:	fascin-1 cDNA
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Very strong	Regionally restricted	2A	Expression is in the mantle layer.
TS17: neural tube	Present		2A
TS17: dorsal root ganglion	Present		2A
TS17: tail somite	Weak		2A
TS17: trunk somite	Weak		2A

Structure	Level	Pattern	Image	Note
TS17: hindbrain mantle layer	Strong	Regionally restricted	2B	Expression is in the myelencephalon.
TS17: trigeminal ganglion	Strong		2B
TS17: 1st branchial arch mandibular component	Strong		2B
TS17: 1st branchial arch maxillary component	Strong		2B
TS17: dorsal root ganglion	Strong		2B
TS17: future spinal cord	Strong		2B
TS17: gut	Present	Regionally restricted	2B	Expression is in the digestive tract.
TS17: liver	Absent		2B
TS17: genitourinary system	Weak		2B

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Strong		2C
TS20: cerebral cortex	Strong		2C
TS20: corpus striatum	Strong		2C
TS20: hypothalamus	Strong		2C
TS20: spinal cord	Strong		2C
TS20: thalamus	Strong		2C
TS20: lateral ganglionic eminence	Strong		2C
TS20: medial ganglionic eminence	Strong		2C
TS20: telencephalon	Strong	Regionally restricted	2C	Expression is in the lateral and medial ganglionic eminences and the amygdala.
TS20: forebrain	Strong	Regionally restricted	2C	Expression is in the hippocampus.
TS20: eye	Strong		2C
TS20: dorsal root ganglion	Strong		2C
TS20: urinary system	Strong		2C
TS20: stomach	Strong	Regionally restricted	2C	Expression is in the stomach wall.
TS20: trunk mesenchyme	Present	Regionally restricted	2C	Expression is in the future intercoastal muscles.
TS20: intraembryonic coelom	Present	Regionally restricted	2C	Expression is in the diaphragm.
TS20: tongue	Present		2C
TS20: gut	Present	Regionally restricted	2C	Expression is in the muscle layer of the gasto-intestinal tract and is weak in the epithelium.
TS20: olfactory epithelium	Weak		2C
TS20: nasal cavity	Strong	Regionally restricted	2C	Expression is in the mesenchyme underlying the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Strong		2D
TS22: cerebral cortex	Strong		2D
TS22: corpus striatum	Strong		2D
TS22: hypothalamus	Strong		2D
TS22: thalamus	Strong		2D
TS22: forebrain	Strong	Regionally restricted	2D	Expression is in the hippocampus.
TS22: lateral ganglionic eminence	Strong		2D
TS22: medial ganglionic eminence	Strong		2D
TS22: telencephalon	Strong	Regionally restricted	2D	Expression is in the lateral and medial ganglionic eminences and the amygdala.
TS22: metanephros	Present		2D
TS22: lung	Weak		2D

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Very strong		2E
TS24: tooth	Very strong		2E
TS24: tongue muscle	Present		2E

Structure	Level	Pattern	Image	Note
TS24: hindlimb musculature	Strong		2F
TS24: hindlimb	Strong	Regionally restricted	2F	Expression is in the tendons and is absent in the cartilagenous primordia.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Strong	Regionally restricted	3A	Expression is in the hippocampus.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Very strong		3B
TS22: spinal cord mantle layer	Very strong		3B

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Strong	Regionally restricted	3C	Expression is in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity	Very strong	Regionally restricted	3D	Expression is in the mesenchyme surrounding the olfactory epithelium.
TS22: olfactory epithelium	Weak		3D

Structure	Level	Pattern	Image	Note
TS22: renal cortex	Strong		3E

Structure	Level	Pattern	Image	Note
TS22: stomach	Strong	Regionally restricted	3F	Expression is in the muscle layers of the gastric wall.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present
TS15: future hindbrain	Present
TS15: future midbrain	Present
TS15: genitourinary system	Present
TS15: lung	Present
TS15: trunk somite	Present
TS15: olfactory placode	Present	Regionally restricted		Expression is in the olfactory epithelium.
TS15: 1st branchial arch mandibular component	Strong
TS15: 1st branchial arch maxillary component	Strong
TS15: 2nd branchial arch	Strong
TS15: dorsal root ganglion	Strong
TS15: facio-acoustic preganglion complex	Strong
TS15: gut	Strong
TS15: nasal process	Strong
TS15: optic cup	Strong
TS15: tail	Strong
TS15: trigeminal preganglion	Strong
TS15: nose	Strong	Regionally restricted		Expression is in the mesenchyme.
TS15: neural tube lateral wall	Present	Regionally restricted		Expression is strong in the mantle layer and weak in the ependymal layer.
TS15: heart	Weak
TS15: tail unsegmented mesenchyme	Weak
TS15: limb	Strong	Regionally restricted		Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Strong
TS17: 1st branchial arch maxillary component	Strong
TS17: 2nd branchial arch	Strong
TS17: 3rd branchial arch	Strong
TS17: acoustic ganglion	Strong
TS17: dorsal root ganglion	Strong
TS17: facial ganglion	Strong
TS17: forebrain	Strong
TS17: glossopharyngeal ganglion	Strong
TS17: gut	Strong
TS17: hindbrain	Strong
TS17: midbrain	Strong
TS17: nasal process	Strong
TS17: optic cup inner layer	Strong
TS17: optic cup outer layer	Strong
TS17: tail	Strong
TS17: trigeminal ganglion	Strong
TS17: vagus ganglion	Strong
TS17: nose	Strong	Regionally restricted		Expression is in the mesenchyme.
TS17: olfactory epithelium	Present	Regionally restricted
TS17: neural tube lateral wall	Present	Regionally restricted		Expression is strong in the mantle layer and weak in the ependymal layer.
TS17: genitourinary system	Present
TS17: lung	Present
TS17: tail somite	Present
TS17: trunk somite	Present
TS17: limb	Strong	Regionally restricted		Expression is in the mesenchyme.
TS17: heart	Weak	Regionally restricted
TS17: liver	Absent

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong
TS20: facial ganglion	Strong
TS20: forebrain	Strong
TS20: glossopharyngeal ganglion	Strong
TS20: hindbrain	Strong
TS20: mandibular process	Strong
TS20: maxillary process	Strong
TS20: midbrain	Strong
TS20: nasal process	Strong
TS20: neural retinal epithelium	Strong
TS20: spinal cord mantle layer	Strong
TS20: tail	Strong
TS20: trigeminal ganglion	Strong
TS20: urinary system	Strong
TS20: vagus ganglion	Strong
TS20: vestibulo-cochlear ganglion complex	Strong
TS20: nose	Strong			Expression is in the mesenchyme.
TS20: lung	Present
TS20: olfactory epithelium	Present
TS20: perioptic mesenchyme	Present
TS20: pigmented retinal epithelium	Present
TS20: skeletal muscle	Present
TS20: heart	Weak
TS20: lens vesicle	Weak
TS20: spinal cord ventricular layer	Weak
TS20: optic stalk	Present	Regionally restricted		Expression is in the optic nerve.
TS20: gut	Present	Regionally restricted		Expression is strong in the muscles, and is moderate in the mucosa.
TS20: stomach	Present	Regionally restricted		Expression is strong in the muscles, and is moderate in the mucosa.
TS20: liver	Absent
TS20: intraembryonic coelom	Strong	Regionally restricted		Expression is in the diaphragm.
TS20: limb	Present	Regionally restricted		Expression is in the muscle.

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Strong
TS22: dorsal root ganglion	Strong
TS22: facial ganglion	Strong
TS22: forebrain	Strong
TS22: glossopharyngeal ganglion	Strong
TS22: hindbrain	Strong
TS22: midbrain	Strong
TS22: neural retinal epithelium	Strong
TS22: spinal cord mantle layer	Strong
TS22: tail	Strong
TS22: tongue muscle	Strong
TS22: tooth	Strong
TS22: trigeminal ganglion	Strong
TS22: urinary system	Strong
TS22: vagus ganglion	Strong
TS22: vestibulo-cochlear ganglion	Strong
TS22: nose	Strong	Regionally restricted		Expression is in the nasal process and mesenchyme.
TS22: lung	Present
TS22: olfactory epithelium	Present
TS22: optic nerve	Present
TS22: perioptic mesenchyme	Present
TS22: pigmented retinal epithelium	Present
TS22: primary palate	Present
TS22: skeletal muscle	Present
TS22: heart	Weak
TS22: lens	Weak
TS22: spinal cord ventricular layer	Weak
TS22: gut	Present	Regionally restricted		Expression is strong in the muscles, and is moderate in the mucosa.
TS22: stomach	Present	Regionally restricted		Expression is strong in the muscles, and is moderate in the mucosa.
TS22: liver	Absent
TS22: limb	Present	Regionally restricted		Expression is in the muscle.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong
TS24: cerebral cortex ventricular layer	Strong
TS24: ganglionic eminence	Present
TS24: cerebellum	Present
TS24: epithalamus	Present
TS24: hypothalamus	Present
TS24: olfactory lobe	Present
TS24: spinal cord	Present
TS24: thalamus	Present
TS24: vertebral axis musculature	Present	Regionally restricted		Expression is in back and trunk muscles.