Mest Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91108 Kaneda M, et al., Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature. 2004 Jun 24;429(6994):900-3
Assay type:	Northern blot
MGI Accession ID:	MGI:3511576
Gene symbol:	Mest
Gene name:	mesoderm specific transcript

Probe:	Peg1 cDNA
Probe preparation:	Double stranded labelled with P32 DNA

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
wt	E10.5 (a)	TS17: embryo	Present	10 µg; total RNA	Not Specified		Not Specified
3	E10.5 (a)	TS17: embryo	Present (b)	10 µg; total RNA	involves: 129S4/SvJae	Dnmt3a^tm3.2Enl/Dnmt3a⁺	Not Specified	embryo's mother was a conditional mutant in the germ line
4	E10.5 (a)	TS17: embryo	Present (b)	10 µg; total RNA	involves: 129S4/SvJae	Dnmt3a^tm3.2Enl/Dnmt3a⁺	Not Specified	embryo's mother was a conditional mutant in the germ line

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels were increased 1.6 fold in the mutants relative to those seen in wild type, suggesting that there is expression from the normally silent maternal allele.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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