Ebf1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91257 Gray PA, et al., Mouse Brain Organization Revealed Through Direct Genome-Scale TF Expression Analysis. Science. 2004 Dec 24;306(5705):2255-2257
Assay type:	RNA in situ
MGI Accession ID:	MGI:3507898
Gene symbol:	Ebf1
Gene name:	early B cell factor 1

Probe:	MTF#690
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Additional electronic submission not included in Supplemental Table 4 of the publication. Results obtained using this probe were the same as probe MTF#372 (MGI:3506165).

Results

Specimen 1: embryonic day 13.5 (more

)

Structure	Level	Pattern	Note
TS21: head	Present		Expression was detected in neural and non-neural tissue.
TS21: central nervous system	Present	Single cells	Expression was detected in some cells.
TS21: cerebral cortex	Present	Single cells	Expression was detected in some cells.
TS21: corpus striatum	Present	Single cells	Expression was detected in some cells.
TS21: thalamus	Present	Single cells	Expression was detected in some cells.
TS21: hypothalamus	Present	Single cells	Expression was detected in some cells.
TS21: midbrain	Present	Single cells	Expression was detected in some cells.
TS21: hindbrain	Present	Single cells	Expression was detected in some cells.
TS21: spinal cord	Present	Single cells	Expression was detected in some cells.
TS21: cranial ganglion	Present	Single cells	Expression was detected in some cells.
TS21: dorsal root ganglion	Present	Single cells	Expression was detected in some cells.
TS21: brain ventricular layer	Absent
TS21: retina	Present	Single cells	Expression was detected in some cells.
TS21: ear	Present	Single cells	The tissue was inner ear and ear structures. Expression was detected in some cells.
TS21: olfactory lobe	Present	Single cells	The tissue was olfactory epithelium and/or bulb. Expression was detected in some cells.

Specimen 2: postnatal newborn (more

)

Structure	Level	Pattern	Image	Note
TS27: head	Present	Regionally restricted	MTF# P 690 Image	Expression was neural specific.
TS27: central nervous system	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: cerebral cortex	Absent		MTF# P 690 Image
TS27: corpus striatum	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: thalamus	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: hypothalamus	Absent		MTF# P 690 Image
TS27: midbrain	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: cerebellum	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: hindbrain	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: spinal cord	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: cranial ganglion	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: dorsal root ganglion	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: brain ventricular layer	Absent		MTF# P 690 Image
TS27: retina	Present	Single cells	MTF# P 690 Image	Expression was detected in some cells.
TS27: ear	Absent		MTF# P 690 Image	The tissue was inner ear and ear structures.
TS27: main olfactory bulb	Present	Single cells	MTF# P 690 Image	The tissue was olfactory epithelium and/or bulb. Expression was detected in some cells.

Specimen S4: postnatal newborn (more

)
Note: Authors did not specify which probe was used when staining this specimen; it is possible MTF#372 (MGI:3506165) was used.

Structure	Level	Pattern	Image	Note
TS27: retina	Present		S4_Ebf1

Specimen S5: postnatal newborn (more

)
Note: Authors did not specify which probe was used when staining this specimen; it is possible MTF#372 (MGI:3506165) was used.

Structure	Level	Pattern	Image	Note
TS27: retina	Present		S5_Olf1

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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