Lhx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91257 Gray PA, et al., Mouse Brain Organization Revealed Through Direct Genome-Scale TF Expression Analysis. Science. 2004 Dec 24;306(5705):2255-2257
Assay type:	RNA in situ
MGI Accession ID:	MGI:3507674
Gene symbol:	Lhx2
Gene name:	LIM homeobox protein 2

Probe:	MTF#364
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Additional electronic submission not included in Supplemental Table 4 of the publication. Results obtained using this probe were the same as probe MTF#277 (MGI:3506131).

Results

Specimen 1: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: head	Present		MTF# E 364 Image	Expression was detected in neural and non-neural tissue.
TS21: central nervous system	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: cerebral cortex	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: corpus striatum	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: thalamus	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: hypothalamus	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: midbrain	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: hindbrain	Absent		MTF# E 364 Image
TS21: spinal cord	Absent		MTF# E 364 Image
TS21: cranial ganglion	Absent		MTF# E 364 Image
TS21: dorsal root ganglion	Absent		MTF# E 364 Image
TS21: brain ventricular layer	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: retina	Present	Single cells	MTF# E 364 Image	Expression was detected in some cells.
TS21: ear	Absent		MTF# E 364 Image	The tissue was inner ear and ear structures.
TS21: olfactory lobe	Present	Single cells	MTF# E 364 Image	The tissue was olfactory epithelium and/or bulb. Expression was detected in some cells.

Specimen 2: postnatal newborn (more

)

Structure	Level	Pattern	Image	Note
TS27: head	Present		MTF# P 364 Image	Expression was detected in neural and non-neural tissue.
TS27: central nervous system	Present	Single cells	MTF# P 364 Image	Expression was detected in some cells.
TS27: cerebral cortex	Present	Single cells	MTF# P 364 Image	Expression was detected in some cells.
TS27: corpus striatum	Absent		MTF# P 364 Image
TS27: thalamus	Present	Single cells	MTF# P 364 Image	Expression was detected in some cells.
TS27: hypothalamus	Present	Single cells	MTF# P 364 Image	Expression was detected in some cells.
TS27: midbrain	Present	Single cells	MTF# P 364 Image	Expression was detected in some cells.
TS27: cerebellum	Absent		MTF# P 364 Image
TS27: hindbrain	Present	Single cells	MTF# P 364 Image	Expression was detected in some cells.
TS27: spinal cord	Absent		MTF# P 364 Image
TS27: cranial ganglion	Absent		MTF# P 364 Image
TS27: dorsal root ganglion	Absent		MTF# P 364 Image
TS27: brain ventricular layer	Absent		MTF# P 364 Image
TS27: retina	Present	Single cells	MTF# P 364 Image	Expression was detected in some cells.
TS27: ear	Absent		MTF# P 364 Image	The tissue was inner ear and ear structures.
TS27: main olfactory bulb	Present	Single cells	MTF# P 364 Image	The tissue was olfactory epithelium and/or bulb. Expression was detected in some cells.

Specimen 1A: postnatal newborn (more

)
Note: Authors did not specify which probe was used when staining this specimen; it is possible MTF#277 (MGI:3506131) was used.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		1A Lhx2

Specimen S3: postnatal newborn (more

)
Note: Authors did not specify which probe was used when staining this specimen; it is possible MTF#277 (MGI:3506131) was used.

Structure	Level	Pattern	Image	Note
TS27: thalamus	Present		S3_Lhx2

Specimen S5: postnatal newborn (more

)
Note: Authors did not specify which probe was used when staining this specimen; it is possible MTF#277 (MGI:3506131) was used.

Structure	Level	Pattern	Image	Note
TS27: retina	Present		S5_Lhx2

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24