Tg(ZP3)7812Dean
Transgene Detail
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| Symbol: |
Tg(ZP3)7812Dean |
| Name: |
transgene insertion 7812, Jurrien Dean |
| MGI ID: |
MGI:3056745 |
| Synonyms: |
huZP3, TgN(HuZP3)3NIH |
| Transgene: |
Tg(ZP3)7812Dean Location: unknown
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| Alliance: |
Tg(ZP3)7812Dean page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(ZP3)7812Dean expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
ZP3 (7784) |
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Mutation details: This transgene comprises 6 kb of 5' flanking DNA from the mouse zona pellucida 3 gene ligated to the 5' half of the human zona pellucida 3 gene, including exons and introns 1-4, followed by cDNA encoded by exons 5-8 of the human gene and the bovine growth hormone polyadenylation signal. This transgenic line was estimated by Southern blot densitometry to carry two copies of the transgene. Transgene expression was shown by RNase protection analysis of multiple tissues to be ovary-specific. In situ hybridization localized expression to the cytoplasm of growing oocytes, at a level similar to that of the co-expressed endogenous mouse gene. Species-specific antibodies detect lower levels of human and mouse ZP3 in zonae pellucidae of mature transgenic oocytes than in human or wild-type mouse zonae, respectively; the total (human + mouse) approximating that in normal mouse zonae. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein.
(J:77459)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Three transgenic lines were established. This line and the line originally called TgN(HuZP3)2NIH each was estimated by Southern blot densitometry to carry 2 copies, and the line originally called TgN(HuZP3)1NIH to carry 4-6 copies of the transgene. Authors state that null-allele rescue results using the former two strains were identical.
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| Original: |
J:77459 Rankin TL, et al., Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development. 1998 Jul;125(13):2415-24 |
| All: |
3 reference(s) |
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Analysis Tools
