Eportm1.1(EGFP/icre)Uk
Targeted Allele Detail
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Symbol: |
Eportm1.1(EGFP/icre)Uk |
Name: |
erythropoietin receptor; targeted mutation 1.1, Ursula Klingmuller |
MGI ID: |
MGI:3052727 |
Synonyms: |
EpoRCre, EpoR-iCre, Eportm1.1(EGFP/icre)Uk, ErGFPcre |
Gene: |
Epor Location: Chr9:21870193-21874802 bp, - strand Genetic Position: Chr9, 7.93 cM
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Alliance: |
Eportm1.1(EGFP/icre)Uk page
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Germline Transmission: |
Earliest citation of germline transmission:
J:92235
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Parent Cell Line: |
BALB/c-I (ES Cell)
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Strain of Origin: |
BALB/cJ
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Allele Type: |
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Targeted (Null/knockout, Recombinase, Reporter) |
Mutation: |
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Insertion
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Eportm1.1(EGFP/icre)Uk expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Epor (MGI:95408) |
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Mutation details: An EGFP/icre sequence was inserted into exon 1, replacing the endogenous ATG with the translational start site of the EGFP/icre, yet preserving the last 10 bp of the splice-donor site of exon 1. A FRT flanked neo was inserted downstream to replace parts of exon 2 and exons 3-8. Transient FLP expression excised the neo. Southern blot and multiplex PCR confirmed recombination in heterozygous mice. Immunoblot of FLCs demonstrated green fluorescence in heterozygous and homozygous embryos, with homozygotes showing a 2-fold increase in fluorescence intensity compared to heterozygotes.
(J:92235)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Epor
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:92235 Heinrich AC, et al., A mouse model for visualization and conditional mutations in the erythroid lineage. Blood. 2004 Aug 1;104(3):659-66 |
All: |
75 reference(s) |
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