Rspo2ftls
Transgenic Allele Detail
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| Symbol: |
Rspo2ftls |
| Name: |
R-spondin 2; footless |
| MGI ID: |
MGI:3045965 |
| Synonyms: |
ftl, Rspo2Tg |
| Gene: |
Rspo2 Location: Chr15:42884190-43034222 bp, - strand Genetic Position: Chr15, 16.73 cM
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| Alliance: |
Rspo2ftls page
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Rspo2ftls/Rspo2ftls Rspo3tm1.1Jcob/Rspo3tm1.2Jcob Tg(Prrx1-cre)1Cjt/0 double mutants have more severe limb defects than single mutants
Show the 2 phenotype image(s) involving this allele.
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: This mutation was caused by the insertion of 6 to 8 copies of a transgenic construct containing the chloramphenicol acetyltransferase (CAT) gene adjoined to a rat H+/K+ ATPase promoter. Approximately 7 kb of genomic DNA was deleted at the insertion site. This insertion/deletion was shown to be located in intron 3 of the Rspo2 gene. No exons of the gene were deleted. Analysis of the transcript by RT-PCR, northern and in situ hybridization analysis showed that a smaller transcript is expressed from this allele that results from the splicing of exon 3 to a cryptic splice site in the inserted transgene. RT-PCR analysis indicates that a very low level of full-length transcript is expressed; however, no full length transcript is detectable by in-situ hybridization. This allele is described as a severe hypomorph.
(J:83881, J:131960)
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| Inheritance: |
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Recessive |
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Rspo2 Mutation: |
19 strains or lines available
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| Original: |
J:83881 Bell SM, et al., Asymmetric limb malformations in a new transgene insertional mutant, footless. Mech Dev. 2003 May;120(5):597-605 |
| All: |
4 reference(s) |
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Analysis Tools
