Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67784
Takeda S, et al., Continuous expression of Cbfa1 in nonhypertrophic chondrocytes uncovers its ability to induce hypertrophic chondrocyte differentiation and partially rescues Cbfa1-deficient mice. Genes Dev. 2001 Feb 15;15(4):467-81
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3044753
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Gene symbol:
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Ihh
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Gene name:
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Indian hedgehog
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Probe:
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Ihh cDNA8
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5A:
embryonic day 16.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: rib |
Absent |
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5A wt Ihh
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Expression was not detected in chondrocostal cartilage.
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Specimen
5B:
postnatal newborn
(more )
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: rib |
Absent |
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5B wt Ihh
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Expression was not detected in chondrocostal cartilage.
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Specimen
7C +/-:
embryonic day 18.5; Runx2tm1Mjo/Runx2 +
(more )
Specimen
7C +/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: skeleton |
Present |
Regionally restricted |
7C Ihh +/-
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Expression was detected in prehypertrophic chondrocytes of long bones (femur or humerus).
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Specimen
7C -/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2tm1Mjo
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: skeleton |
Absent |
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7C Ihh -/-
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Expression was not detected in prehypertrophic chondrocytes of long bones (femur or humerus).
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Analysis Tools
