Probe:	Wnt5a cDNA6
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The majority of the data are not shown. The lens development data is summarized in Table 1. The authors state that expression for this gene was generally similar at all ages as was described for Wnt7a (as shown in MGI:3041710). Notable differences in expression as stated by the authors are described below.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Weak

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Weak	Regionally restricted	1J	Expression is absent in the central epithelium and is weak in the equatorial region.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Weak	Regionally restricted	1J1	Expression is absent in the central epithelium and is weak in the equatorial region.

Structure	Level	Pattern	Image	Note
TS15: future brain	Weak
TS15: head mesenchyme	Weak
TS15: surface ectoderm	Weak	Regionally restricted		Expression is in the ectoderm of the head.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present
TS17: optic cup	Present	Regionally restricted		Expression is in the retinal primordia.
TS17: brain	Weak
TS17: head mesenchyme	Weak
TS17: surface ectoderm	Weak	Regionally restricted		Expression is in the ectoderm of the head.

Structure	Level	Pattern	Image	Note
TS20: cornea	Present
TS20: hyaloid vascular plexus	Present
TS20: lens vesicle	Strong	Regionally restricted		Expression is in the elongating primary fibers.
TS20: lens vesicle epithelium	Strong

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Strong	Regionally restricted		Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS22: cornea epithelium	Strong
TS22: lens epithelium	Strong
TS22: cornea stroma	Present
TS22: eye	Present	Regionally restricted		The strongest expression is in the margin in the cells destined to form the ciliary and iridial epithelia.
TS22: hyaloid vascular plexus	Weak

Structure	Level	Pattern	Image	Note
TS23: lens fiber	Strong	Regionally restricted		Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS23: cornea epithelium	Strong
TS23: lens epithelium	Strong
TS23: cornea stroma	Present
TS23: eye	Present	Regionally restricted		The strongest expression is in the margin in the cells destined to form the ciliary and iridial epithelia.
TS23: hyaloid vascular plexus	Weak

Structure	Level	Pattern	Image	Note
TS26: lens fiber	Strong	Regionally restricted		Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS26: cornea epithelium	Strong
TS26: lens epithelium	Strong
TS26: cornea stroma	Present
TS26: eye	Present	Regionally restricted		The strongest expression is in the margin in the cells destined to form the ciliary epithelia.
TS26: iris	Present	Regionally restricted		Expression is in the iridial epithelium.
TS26: hyaloid vascular plexus	Weak

Structure	Level	Pattern	Image	Note
TS27: cornea endothelium	Strong
TS27: cornea epithelium	Strong
TS27: lens epithelium	Strong
TS27: ciliary body	Present
TS27: iris	Present

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Strong
TS28: cornea epithelium	Strong
TS28: lens epithelium	Strong
TS28: ciliary body	Present
TS28: iris	Present

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Strong
TS28: cornea epithelium	Strong
TS28: ciliary body	Present
TS28: iris	Present