Probe:	Wnt7a cDNA6
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The lens development data is summarized in Table 1.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Weak

Structure	Level	Pattern	Image	Note
TS15: future brain	Weak		1A1
TS15: head mesenchyme	Weak		1A1
TS15: surface ectoderm	Weak	Regionally restricted	1A1	Shown is expression in the ectoderm of the head.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present		1B
TS16: optic cup	Present	Regionally restricted	1B	Expression is in the retinal primordia.
TS16: future brain	Weak		1B
TS16: head mesenchyme	Weak		1B
TS16: surface ectoderm	Weak	Regionally restricted	1B	Shown is expression in the ectoderm of the head.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present		1B1
TS16: optic cup	Present	Regionally restricted	1B1	Expression is in the retinal primordia.
TS16: future brain	Present		1B1
TS16: head mesenchyme	Present		1B1
TS16: surface ectoderm	Present	Regionally restricted	1B1	Shown is expression in the ectoderm of the head.

Structure	Level	Pattern	Image	Note
TS20: cornea	Present		1C
TS20: hyaloid vascular plexus	Present		1C
TS20: lens vesicle	Strong	Regionally restricted	1C	Expression is in the elongating primary fibers.
TS20: lens vesicle epithelium	Strong		1C

Structure	Level	Pattern	Image	Note
TS20: cornea	Present		1C1
TS20: hyaloid vascular plexus	Present		1C1
TS20: lens vesicle	Strong	Regionally restricted	1C1	Expression is in the elongating primary fibers.
TS20: lens vesicle epithelium	Strong		1C1

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Strong	Regionally restricted	1D	Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS22: cornea epithelium	Strong		1D
TS22: lens epithelium	Strong		1D
TS22: cornea stroma	Present		1D
TS22: eye	Present	Regionally restricted	1D	The strongest expression is in the margin in the cells destined to form the ciliary and iridial epithelia.
TS22: hyaloid vascular plexus	Weak		1D

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Strong	Regionally restricted	1D1	Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS22: cornea epithelium	Strong		1D1
TS22: lens epithelium	Strong		1D1
TS22: cornea stroma	Present		1D1
TS22: eye	Present	Regionally restricted	1D1	The strongest expression is in the margin in the cells destined to form the ciliary and iridial epithelia.
TS22: hyaloid vascular plexus	Weak		1D1

Structure	Level	Pattern	Image	Note
TS23: lens fiber	Strong	Regionally restricted	1E	Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS23: cornea epithelium	Strong		1E
TS23: lens epithelium	Strong		1E
TS23: cornea stroma	Present		1E
TS23: eye	Present	Regionally restricted	1E	The strongest expression is in the margin in the cells destined to form the ciliary and iridial epithelia.
TS23: hyaloid vascular plexus	Weak		1E

Structure	Level	Pattern	Image	Note
TS23: lens fiber	Strong	Regionally restricted	1E1	Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS23: cornea epithelium	Strong		1E1
TS23: lens epithelium	Strong		1E1
TS23: cornea stroma	Present		1E1
TS23: eye	Present	Regionally restricted	1E1	The strongest expression is in the margin in the cells destined to form the ciliary and iridial epithelia.
TS23: hyaloid vascular plexus	Weak		1E1

Structure	Level	Pattern	Image	Note
TS26: lens fiber	Strong	Regionally restricted	1F	Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS26: cornea epithelium	Strong		1F
TS26: lens epithelium	Strong		1F
TS26: cornea stroma	Present		1F
TS26: eye	Present	Regionally restricted	1F	The strongest expression is in the margin in the cells destined to form the ciliary epithelia.
TS26: iris	Present	Regionally restricted	1F	Expression is in the iridial epithelium.
TS26: hyaloid vascular plexus	Weak		1F

Structure	Level	Pattern	Image	Note
TS26: lens fiber	Strong	Regionally restricted	1F1	Expression is in the less mature elongating fibers at the lens equator and is absent in the more mature fibers in the center of the lens.
TS26: cornea epithelium	Strong		1F1
TS26: lens epithelium	Strong		1F1
TS26: cornea stroma	Present		1F1
TS26: eye	Present	Regionally restricted	1F1	The strongest expression is in the margin in the cells destined to form the ciliary epithelia.
TS26: iris	Present	Regionally restricted	1F1	Expression is in the iridial epithelium.
TS26: hyaloid vascular plexus	Weak	Regionally restricted	1F1

Structure	Level	Pattern	Image	Note
TS27: cornea endothelium	Strong		1G
TS27: cornea epithelium	Strong		1G
TS27: lens epithelium	Strong		1G
TS27: ciliary body	Present		1G
TS27: iris	Present		1G

Structure	Level	Pattern	Image	Note
TS27: cornea endothelium	Strong		1G1
TS27: cornea epithelium	Strong		1G1
TS27: lens epithelium	Strong	Graded	1G1	Expression is strongest at the equator.
TS27: ciliary body	Present		1G1
TS27: iris	Present		1G1

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Strong		1H
TS28: cornea epithelium	Strong		1H
TS28: lens epithelium	Strong		1H
TS28: ciliary body	Present		1H
TS28: iris	Present		1H

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Strong		1H1
TS28: cornea epithelium	Strong		1H1
TS28: lens epithelium	Strong	Graded	1H1	Expression is strongest at the equator.
TS28: ciliary body	Present		1H1
TS28: iris	Present		1H1

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Strong	Graded	1I	Expression is strongest at the equator and weaker in the central epithelium.
TS28: cornea endothelium	Strong		1I
TS28: cornea epithelium	Strong		1I
TS28: ciliary body	Present		1I
TS28: iris	Present		1I

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Strong		1I1
TS28: cornea epithelium	Strong		1I1
TS28: lens epithelium	Strong		1I1
TS28: ciliary body	Present		1I1
TS28: iris	Present		1I1